The Effect of Dracocephalum Kotschyi Hydroalcoholic Extract on Biochemical and Hematological Parameters in Rat

Document Type : Original Article (s)

Authors

1 Pharmacy Student, School of Pharmacy and Pharmaceutical Science, Isfahan University of Medical Sciences, Isfahan, Iran

2 Assistant Professor, Department of Clinical Biochemistry, School of Pharmacy and Pharmaceutical Science, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Department of Pharmacognosy, School of Pharmacy and Pharmaceutical Sciences, Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

4 Associate Professor, Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran

5 Associate Professor, Department of Pathology, School of Medicine, Water and Electrolytes Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

6 Associate Professor Department of Pharmacology and Toxicology, School of Pharmacy and Pharmaceutical Sciences, Pharmaceutical Sciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Dracocephalum kotschyi is one of the native medicinal plants of Iran with anti-inflammatory and spasmolytic properties. The aim of this study was to investigate the effects of D. kotschyi hydroalcoholic extract on blood biochemical and hematological parameters in rat.
Methods: In this study, hydroalcoholic extract was prepared by maceration technique and its phenolic content was assessed by Folin-Ciocalteu method. The extract was then administered orally for 30 days (50, 100 &
200 mg/kg) in 3 different rat groups. A normal control group and vehicle treated group were also included for comparison. After a month of extract administration and qualitative observations, blood samples were taken from all the rats for quantitative determination of biochemical and hematological parameters. The liver and kidney sections were also prepared for microscopic pathological examination.
Findings: According to our findings, oral consumption of hydroalcoholic extract of D. kotschyi over a month induced no substantial changes in the measured biochemical and hematological parameters. The high dose of hydroalcoholic extract of D. kotschyi caused a slight increase in the amount of some liver enzymes (AST, ALT and ALP) and the dose of 50 mg/kg elevated creatinine levels. However, examination of liver and kidney sections showed no microscopic tissue damage.
Conclusion: Short term consumption of hydroalcoholic extract of D. kotschyi has no adverse effect on blood biochemical and hematological parameters. However, as long as the long term effect of the extract on liver and renal function is not fully understood, consumption of D. kotschyi in patients with hepatic or renal failure is
not recommended.

Keywords

References

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Journal of Isfahan Medical School
Volume 40, Issue 669
1st Week, July
July and August 2022
Pages 278-287

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History

  • Receive Date: 11 July 2022
  • Accept Date: 11 July 2022

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