Evaluating the Effect of IL-27-Transfected Mesenchymal Stem Cells on Certain Histological and Immunological Parameters in a Mouse Model of Experimental Autoimmune Encephalomyelitis

Document Type : Original Article (s)

Authors

1 Assistant Professor, Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

2 Associate Professor, Department of Anatomy, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran

3 Assistant Professor, Department of Anatomical Sciences and Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Assistant Professor, Department of Physiology and Pharmacology, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran

Abstract

Background: Mesenchymal stem cells (MSCs) by having immunomodulatory and neuroprotectivity properties could be a suitable candidate for the treatment of inflammatory and neurodegenerative diseases. Furthermore, by genetical manipulation, it is possible to potentiate the therapeutic characteristics of these cells. The aim of this study was to investigate the effect of IL-27-transfected MSCs in a mouse model of experimental autoimmune encephalomyelitis (EAE).Methods: MSCs were isolated from the bone marrow of C57BL/6 mice and were trnasfected with mouse IL-27 gene by using a lentiviral vector system. On days 15 and 22 post EAE induction, one million of these cells were injected to EAE-affected mice. Finally, all the mice were sacrificed; and histological and immunological assays were performed to investigate the myelin status and infiltration rate of immune cells into the central nervous system (CNS) tissues and measure certain cytokines, respectively.Findings IL-27-transfected MSCs could ameliorate disease in the EAE-affected mice. Besides, they could diminish the infiltration rate of immune cells into the CNS, and inhibited the myelin destruction. Moreover, IL-27- transfected MSCs could enhance IL-4 and IL-10, and reduce IL-17 production from the EAE mice splenocytes.Conclusion: IL-27-transfected MSCs could be considered as a therapeutic tool for the treatment of inflammatory diseases including multiple sclerosis (MS). Changing dose and rout of administration, however, would be suggested to achieve better results. 

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 249 - Serial Number 249
July and August 2013
Pages 1270-1284

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History

  • Receive Date: 18 May 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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