Diagnosis of Brucellosis via BACTEC Blood Culture System

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Infectious Diseases, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

2 Shahid Beheshti Hospital, Kashan University of Medical Sciences, Kashan, Iran

3 MSc Student, Department of Research and Production of Poultry Viral Vaccine, Razi Vaccine and Serum Producing Research Institute, Karaj, Iran

Abstract

Background: Brucellosis is a zoonosis infectious disease and endemic in Iran. The certain diagnosis of disease is established on isolation of brucella from blood and other clinical samples. So, because of fastidious nature of this organism and problems in its isolation by conventional methods, and attending to the high prevalence of brucellosis in Kashan, Iran, the BACTEC9050 blood culture system was used in this survey for diagnosis of brucella bacteremia; advantages of this method has been discussed, too.Methods: In this descriptive research, the blood samples of 206 patients suspected to brucellosis were studied in Brain-Heart Infusion (BHI) broth and BACTEC9050 blood culture system simultaneously.Findings: In a period of 5 days, from 206 samples, totally 50 cases were positive; 32 cases were positive in both methods and 18 cases were positive only in BACTEC method. Continuing the incubation, 14 cases became positive but 4 cases were negative, even after 30 days of incubation. The average incubation period for specimens to become positive was 4 days.Conclusion: The BACTEC automatic system reduced the examination process, and economized the time and the material. In cases that this system is not available for diagnosis of brucella, it is recommended to keep the final subcultures in 37°C for 1 month to get better diagnostic results.  

Keywords

References

  1. Pathak AD, Dubal ZB, Doijad S, Raorane A, Rodrigues S, Naik R, et al. Human brucellosis among pyrexia of unknown origin cases and occupationally exposed individuals in Goa Region, India. Emerg Health Threats J 2014; 7: 23846.
  2. Sathyanarayanan V, Razak A, Saravu K, Ananthakrishna SB, Mukhyprana PM, Vandana KE. Clinical profile of brucellosis from a tertiary care center in southern India. Asian Pac J Trop Med 2011; 4(5): 397-400.
  3. Bannatyne RM, Jackson MC, Memish Z. Rapid diagnosis of Brucella bacteremia by using the BACTEC 9240 system. J Clin Microbiol 1997; 35(10): 2673-4.
  4. Corbel MJ. Brucellosis in humans and animals. Geneva, Switzerland: World Health Organization; 2006. p. 1-3.
  5. Mantur BG, Biradar MS, Bidri RC, Mulimani MS, Veerappa, Kariholu P, et al. Protean clinical manifestations and diagnostic challenges of human brucellosis in adults: 16 years' experience in an endemic area. J Med Microbiol 2006; 55(Pt 7): 897-903.
  6. Pappas G, Akritidis N, Bosilkovski M, Tsianos E. Brucellosis. N Engl J Med 2005; 352(22): 2325-36.
  7. Mantur BG, Mulimani MS, Bidari LH, Akki AS, Tikare NV. Bacteremia is as unpredictable as clinical manifestations in human brucellosis. Int J Infect Dis 2008; 12(3): 303-7.
  8. Yagupsky P. Detection of Brucella melitensis by BACTEC NR660 blood culture system. J Clin Microbiol 1994; 32(8): 1899-901.
  9. Baysallar M, Aydogan H, Kilic A, Kucukkaraaslan A, Senses Z, Doganci L. Evaluation of the BacT/ALERT and BACTEC 9240 automated blood culture systems for growth time of Brucella species in a Turkish tertiary hospital. Med Sci Monit 2006; 12(7): BR235-BR238.
  10. Ayaslioglu E, Kilic D, Kaygusuz S, Kucuk S, Ceken S, Erol O, et al. The detection of Brucella spp by BACTEC 9050 blood culture system. Mikrobiyol Bul 2004; 38(4): 415-9. [In Turkish].
  11. Barati M, Noorbakhsh S, Bageri Hoseini H, Mortazavi HR. BACTEC medium: a useful method for detection of microorganisms in sterile body fluids. Tehran Univ Med J 2008; 66(5): 305-10. [In Persian].
  12. Maleknejad P, Peeri-DoGaheh H, Amir Zargar AA, Jafari S, Fatollahzadeh B. Diagnosis of brucellosis by use of BACTEC blood culture and confirmation by PCR. J Vet Res 2007; 62(4): 83-6.
  13. Akcam FZ, Yayli G, Uskun E, Kaya O, Demir C. Evaluation of the BACTEC microbial detection system for culturing miscellaneous sterile body fluids. Res Microbiol 2006; 157(5): 433-6.
  14. Maleknejad P, Hashemi FB, Fatollahzadeh B, Jafari S, Peeri Dogaheh H. Direct urease test and acridine orange staining on BACTEC blood culture for rapid presumptive diagnosis of brucellosis. Iran J Public Health 2005; 34(3): 52-5.
  15. Iseri S, Bulut C, Yetkin MA, Kinikli S, Demiroz AP, Tulek N. Comparison of the diagnostic value of blood and bone marrow cultures in brucellosis. Mikrobiyol Bul 2006; 40(3): 201-6. [In Turkish].
  16. Cetin ES, Kaya S, Demirci M, Aridogan BC. Comparison of the BACTEC blood culture system versus conventional methods for culture of normally sterile body fluids. Adv Ther 2007; 24(6): 1271-7.
  17. Al-Attas RA, Al-Khalifa M, Al-Qurashi AR, Badawy M, Al-Gualy N. Evaluation of PCR, culture and serology for the diagnosis of acute human brucellosis. Ann Saudi Med 2000; 20(3-4): 224-8.
Journal of Isfahan Medical School
Volume 32, Issue 315 - Serial Number 315
November and December 2015
Pages 2234-2240

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History

  • Receive Date: 06 January 2015
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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