The Association of A/G Polymorphism in Intronic Region of FGFR2 Gene and Breast Cancer

Document Type : Original Article (s)

Authors

1 Associate Professor, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran

2 MSc Student, Department of Biology, School of Sciences, University of Isfahan, Isfahan, Iran

Abstract

Background: Fibroblast growth factor receptor 2 (FGFR2) is a receptor of tyrosine kinase with a pivotal role in the cell growth and differentiation. FGFR2 gene was identified as susceptibility gene for breast cancer by Genome-wide associated study. rs1219648 polymorphisms in intronic region are associated with breast cancer. FGFR2 gene is amplified in 15-10% of breast tumors. Single-nucleotide polymorphisms (SNPs) of this region are involved in FGFR2 amplification. In this study, the association of rs1219648 in intron 2 region of FGFR2 gene and breast cancer was assessed.Methods: In the present study, 80 cases of breast cancer and 100 healthy controls were studied. After DNA extraction from blood, specific sequence was amplified by tetra primer ARMS-PCR (amplification-refractory mutation system-polymerase chain reaction) technique and genotype of C/T polymorphism was determined by agarose gel electrophoresis.Findings: Individuals with G/G and A/G genotype were at a significantly higher risk of breast cancer (OR = 5.32, P = 0.018). G allele frequency in case patients were greater than controls but this increase did not show significant relationship with breast cancer (P = 0.230).Conclusion: Single nucleotide polymorphism of G/A in intron 2 of the FGFR2 tyrosine kinase receptor gene may play a role as a risk factor for breast cancer susceptibility.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 279 - Serial Number 279
January and February 2014
Pages 359-367

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History

  • Receive Date: 18 December 2013
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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