Effect of varicocelectomy on semen parameters and protamine deficiency 3 and 6 months post surgery

Document Type : Original Article (s)

Authors

1 Department of Biology, Payame Noor University, Isfahan

2 Department of Embryology and Andrology, Royan Institute, Isfahan

3 Department of Anatomy, School of Medicine, Isfahan Medical University, Isfahan

4 Isfahan Fertility and Infertility Center, Isfahan

Abstract

Different methods have been used to evaluate the beneficial effect of varicocelectomy; these include semen parameters and pregnancy rate. Due to high biological variability of semen parameters, sperm functional tests have been considered as an efficient end point in assessment of fertility. Therefore, the aim of this study was to evaluate the effect of varicocelectomy on semen parameters and sperm protamine deficiency. Background: Semen samples were collected from 192 patients by masturbation after 3-4 days of abstinence used for semen analysis and assessment of sperm morphology, chromatin status using Chromomycin A3 and Papanicolaou staining respectively. Methods: The results of present study show that all the 3 semen parameters and percentage of sperm with normal protamine content have improved post surgery (p