The Effect of the Alcoholic Extract of Black Cumin (Nigella Sativa) on Stone Size in Patients with Oxalate Calcium Kidney Stones

Authors

1 MSc Student, Children Growth Research Center, Research Institute for Prevention of Non Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran

2 MD, Department of Nephrology, Velayat Hospital, Qazvin, Iran

3 Associate Professor, Social Determinants of Health Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

4 Assistant Professor, Cellular and Molecular Research Center, Research Institute for Prevention of Non Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran

5 Professor, Children Growth Research Center, Research Institute for Prevention of Non Communicable Diseases, Qazvin University of Medical Sciences, Qazvin, Iran

Abstract

Background: Nephrolithiasis (kidney stone) is a systemic disease associated with metabolic disorders. Regarding the beneficial properties of Nigella sativa, including diuretic and its inhibitory effects of calcium oxalate crystals deposition, this study aimed to investigate the effect of the alcoholic extract of Nigella sativa on kidney stone size in patients.
Methods: This study was a clinical trial conducted from June 2020 to December 2021 in Qazvin, Iran.
50 patients with kidney stones were allocated to two groups using the randomized block sampling method. For 12 weeks, the intervention group received a capsule containing alcoholic extract Nigella sativa (100 mg) two times per day and the control group received their usual treatment. pH, urinary oxalate and citrate, the size of the stone (sonography) were measured before and after the intervention. Paired t-test and independent t-test were used to compare the quantitative mean of the data.
Findings: Despite the decrease in stone size in the intervention group, no statistical significance in difference between both groups was observed. The amount of urinary oxalate in the intervention group was significantly reduced compared to the control group. There were no significant differences in terms of urine volume, pH, citrate levels between the two groups.
Conclusion: Findings of this study showed that alcoholic extract of Nigella sativa can be effective to prevent the accumulation of calcium oxalate crystals by reducing urinary oxalate. Although a complete understanding of the mechanism is unknown.
 

Keywords

References

  1. Mohammadi M, Emami SA, Mahmoudi F, Bahrani S. The effect of preoperative education on postoperative pain and anxiety in patients undergoing percutaneous nephrolithotomy (PCNL). J Isfahan Med Sch 2017; 35(426): 428-33. [In Persian].
  2. Mohammadjafari H, Alipour A, Alaee A, Ahangardarabi S. The incidence and risk factors for urinary stone formation in infants with prenatal history of hydronephrosis. J Isfahan Med Sch 2015; 32(318): 2367-77. [In Persian].
  3. Khalili P, Jamali Z, Sadeghi T, Esmaeili-Nadim A, Mohamadi M, Moghadam-Ahmadi A, et al. Risk factors of kidney stone disease: a cross-sectional study in the southeast of Iran. BMC Urol 2021; 21(1):
  4. Maddahi NS, Mirzaei K, Aghamir SMK, Modaresi SS, Yekaninejad MS. Major dietary patterns and kidney stone formation among Iranian men. J Nutr Sci & Diet 2017; 3(3): 11-7.
  5. Yousefichaijan P, Rezagholi Zamenjany M, Dorreh F, Nakhaei MR, Rafiei M, Babayikazir M. Evaluation of nutritional actors in kidney stones formation in children. Iran J Pediatr 2018; 28(2): e9196.
  6. Cheraghi Nirumand M, Hajialyani M, Rahimi R, Farzaei MH, Zingue S, Nabavi SM, et al. Dietary plants for the prevention and management of kidney stones: preclinical and clinical evidence and molecular mechanisms. Int J Mol Sci 2018; 19(30): 765.
  7. Hayatdavoudi P, Khajavi Rad A, Rajaei Z, Hadjzadeh MAR. Renal injury, nephrolithiasis and Nigella sativa: A mini review. Avicenna J Phytomed 2016; 6(1): 1-8.
  8. Paseban M, Niazmand S, Soukhtanloo M, Meybodi NT. The preventive effect of Nigella sativa seed on gastric ulcer induced by indomethacin in rat. J Herbmed Pharmacol 2020; 9(1): 12-9.
  9. Bahmani M, Baharvand-Ahmadi B, Tajeddini P, Rafieian-Kopaei M, Naghdi N. Identification of medicinal plants for the treatment of kidney and urinary stones. J Renal Inj Prev 2016; 5(3): 129-33.
  10. Hannan MA, Zahan MS, Sarker PP, Moni A, Ha H, Uddin MJ. Protective effects of black cumin (Nigella sativa) and its bioactive constituent, thymoquinone against kidney injury: An aspect on pharmacological insights. Int J Mol Sci 2021; 22(16): 9078.
  11. Zarei M, Rahmani Z, Rezazadeh J, Hadjzadeh MAR. Study of preventive effectiveness of Nigella sativa L. against rat kidney stones, generated by ethylene Glycol. Br J Pharm Res 2015; 8(6): 1-7.
  12. Hajzadeh M, Khouei A, Hajzadeh Z, Parizady M. Ethanolic extract of nigella sativa L seeds on ethylene glycol-induced kidney calculi in rats. Urol J 2007; 4(2): 86-90.
  13. Ardakani Movaghati MR, Yousefi M, Saghebi SA, Sadeghi Vazin M, Iraji A, Mosavat SH. Efficacy of black seed (Nigella sativa L.) on kidney stone dissolution: A randomized, double‐blind, placebo‐controlled, clinical trial. Phytother Res 2019; 33(5):
    1404-12.
  14. Benhelima A, Kaid-Omar Z, Hemida H, Benmahdi T, Addou A. Nephroprotective and diuretic effect of Nigella sativa L seeds oil on lithiasic wistar rats. Afr J Tradit Complement Altern Med 2016; 13(6): 204-14.
  15. Hadjzadeh M, Khajavi Rad A, Rajaei Z, Tehranipour M, Monavar N. The preventive effect of N-butanol fraction of Nigella sativa on ethylene glycol-induced kidney calculi in rats. Pharmacogn Mag 2011; 7(28): 338-43.
  16. Afkari R, Feizabadi MM, Ansari-Moghadam A, Safari T, Bokaeian M. Simultaneous use of oxalate-degrading bacteria and herbal extract to reduce the urinary oxalate in a rat model: A new strategy. Int Braz J Urol 2019; 45(6): 1249-59.
  17. Phillips R, Hanchanale VS, Myatt A, Somani B, Nabi G, Biyani CS. Citrate salts for preventing and treating calcium containing kidney stones in adults. Cochrane Database Syst Rev 2015; 6(10): CD010057.
  18. Han H, Segal AM, Seifter JL, Dwyer JT. Nutritional management of kidney stones (nephrolithiasis). Clin Nutr Res 2015; 4(3): 137-52.
  19. Nikpay S, Moradi K, Azami M, Babashahi M, Otaghi M, Borji M. Frequency of kidney stone different compositions in patients referred to a lithotripsy center in Ilam, west of Iran. J Ped Nephrol 2016; 4(3): 102-7.
  20. Akhavan Sepahi M, Hosini R. Approach and medical management of urinary tract stone in children. Caspian J Pediatr March 2016; 2(1): 86-95.
  21. Hosseinian S, Ebrahimzadeh Bideskan A, Shafei MN, Sadeghnia HR, Soukhtanloo M, Shahraki S, et al. Nigella sativa extract is a potent therapeutic agent for renal inflammation, apoptosis, and oxidative stress in a rat model of unilateral ureteral obstruction. Phytother Res 2018; 32(11): 2290-8.
  22. Asadi-Samani M, Kafash-Farkhad N, Azimi N, Fasihi A, Alinia-Ahandani E, Rafieian-Kopaei M. Medicinal plants with hepatoprotective activity in Iranian folk medicine. Asian Pac J Trop Biomed 2015; 5(2): 146-57.
  23. Shirani M, Arjaki D, Kheiri S, Bijad E, Mohammadi S, Lorigooini Z. An in vitro screening potential traditional medicinal plants for nephrolithiasis. Clin Phytosci 2020; 6:
  24. Ahmed S, Mohtasheemul Hasan M, Khan H, Alam Mahmood Z, Patel S. The mechanistic insight of polyphenols in calcium oxalate urolithiasis mitigation. Biomed Pharmacother 2018; 106: 1292-9.
  25. El-Dakhakhny M, Madi NJ, Lembert N, Ammon HPT. Nigella sativa oil, nigellone and derived thymoquinone inhibit synthesis of 5-lipoxygenase products in polymorphonuclear leukocytes from rats. J Ethnopharmacol 2002; 81(2): 161-4.
  26. Bashir S, Gilani AH, Siddiqui AA, Pervez S, Khan SR, Sarfaraz NJ, et al. Berberis vulgaris root bark extract prevents hyperoxaluria induced urolithiasis in rats. Phytother Res 2010; 24(8): 1250-5.
Journal of Isfahan Medical School
Volume 40, Issue 661
1st Week, May
May and June 2022
Pages 103-108

Files

History

  • Receive Date: 02 May 2022
  • Accept Date: 02 May 2022

Share

How to cite

Statistics