In-vitro Toxicity Assessment of Polydopamine-Coated and Uncoated Fe3O4 Nanoparticles in Cell Line B16-F10 (Melanoma Cell)

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Cellular and Molecular Biology (Biophysics), Falavarjan Branch, Islamic Azad University, Isfahan, Iran

2 Assistant Professor, Department of Cellular and Molecular Biology (Biophysics), Falavarjan Branch, Islamic Azad University, Isfahan, Iran

3 Professor, Department of Medical Physics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: In recent years, due to their small size and increased surface-to-volume ratio, the use of nanoparticles in diagnosis, drug delivery, and treatment has received much attention. The most important problem in chemotherapy is the limited access to the central part of the tumor due to its low blood flow. The aim of this study was to investigate the toxicity of polydopamine-coated and uncoated Fe3O4 nanoparticles on melanoma B16-F10 cells.Methods: Fe3O4 nanoparticles were co-precipitated and coated with dopamine. Then, the effect of cytotoxicity of the coated and uncoated nanoparticles on the B16-F10 cells was studied using MTT assay.Findings: Uncoated Fe3O4 nanoparticles were toxic to B16-F10 at the concentration of 450 μg/ml and duration of 72 hours. Coated nanoparticles had no toxicity at any concentration at the duration of 48 and 72 hours.Conclusion: The cytotoxic properties of uncoated nanoparticles were significantly higher than the coated nanoparticles. Polydopamine coating shell can reduce the toxicity of the nanoparticles, and it can be used for bonding the drugs, as well as a contrast agent in magnetic resonance imaging (MRI).

Keywords

References

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Journal of Isfahan Medical School
Volume 37, Issue 533 - Serial Number 533
May and June 2019
Pages 762-767

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History

  • Receive Date: 12 August 2019
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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