The Effects of An Acute Running Exercise Training Session on Changes in Serum Beta-Endorphin and Cortisol Levels among Male Sprint Runners

Document Type : Original Article (s)

Authors

1 Department of Physical Education and Sport Sciences, Khorasgan Branch, Islamic Azad University, Isfahan, Iran

2 Associate Professor, Department of Exercise Physiology, School of Physical Education and Sport Sciences, The University of Isfahan, Isfahan, Iran

3 Associate Professor, Department of Pathology, Isfahan University of Medical Sciences, Isfahan, Iran

4 Assistant Professor, Department of Physiology, School of Physical Education and Sport Sciences, Khorasgan Branch, Islamic Azad University, Isfahan, Iran

5 General Practitioner, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Previous studies have often focused on plasma beta-endorphin (ß-EP) and cortisol responses to either sub-maximal or maximal exercise. However, little research has been conducted on the effects of professional exercise sessions on ß-EP and cortisol secretion levels. The aim of this study was to assess changes in plasma ß-EP and cortisol levels in male sprint runners after one session of 200 meters run exercise training.Methods: In a quasi-experimental study, 14 healthy male elite runners (mean age: 21.38 ± 2.76 years, mean weight: 69.78 ± 6.76 kg, mean height: 182.21 ± 5.22 cm, and mean body mass index of 20.99 ± 1.62 kg/m2) purposefully and voluntarily participated in this study. After a two hour exercise session, the subjects ran 200 meters in 23 seconds with an intensity equivalent to 80-85% of maximum oxygen consumption. Venous blood samples were taken from the right arm of the subjects to measure levels of ß-EP and cortisol before exercise, immediately after specialized training, and 30 minutes after exercise. Serum blood samples were separated immediately in a refrigerated centrifuge at +4°C. They were then transferred to a -70°C refrigerator for future assessments. ß-EP and cortisol were measured using enzyme-linked immunosorbent assay (ELISA) method. Data was analyzed using analysis of variance (ANOVA).Findings: Levels of ß-EP among male sprint runners significantly differed between the three measurements (P ≤ 0.05). The paired comparison test showed a significant difference between the mean ß-EP level before training and in the recovery period (P ≤ 0.05). In addition, no significant differences in cortisol levels were observed (P ≥ 0.05).Conclusion: The results showed that an acute exercise session caused significant increases only in levels of serum ß-EP hormones among sprint runners.

Keywords

References

  1. Farrell PA, Gates WK, Maksud MG, Morgan WP. Increases in plasma beta-endorphin/
  2. beta-lipotropin immunoreactivity after treadmill running in humans. Journal of Applied Physiology 1982; 52(5): 1245-9.
  3. de MK, Naaktgeboren N, Van SA, Gorus F, Olbrecht J, Block P. Beta-endorphin and ACTH levels in peripheral blood during and after aerobic and anaerobic exercise. Eur J Appl Physiol Occup Physiol 1986; 55(1): 5-8.
  4. Bender T, Nagy G, Barna I, Tefner I, Kadas E, Geher P. The effect of physical therapy on beta-endorphin levels. Eur J Appl Physiol 2007; 100(4): 371-82.
  5. Kraemer RR, Kilgore JL, Kraemer GR, Castracane VD. Growth hormone, IGF-I, and testosterone responses to resistive exercise. Med Sci Sports Exerc 1992; 24(12): 1346-52.
  6. Rasaee MG, Gaeeni AA, Nazem F. Hormone compliance and exercise. 1st ed. Tehran: University Of Tarbiyat Modaress; 2004.
  7. Kraemer RR, Blair S, Kraemer GR, Castracane VD. Effects of treadmill running on plasma beta-endorphin, corticotropin, and cortisol levels in male and female 10K runners. Eur J Appl Physiol Occup Physiol 1989; 58(8): 845-51.
  8. Fazio E, Medica P, Aronica V, Grasso L, Ferlazzo A. Circulating beta-endorphin, adrenocorticotrophic hormone and cortisol levels of stallions before and after short road transport: stress effect of different distances. Acta Vet Scand 2008; 50: 6.
  9. Boecker H, Sprenger T, Spilker ME, Henriksen G, Koppenhoefer M, Wagner KJ, et al. The Runner's High: Opioidergic Mechanisms in the Human Brain. Cereb Cortex 2008; 18(11): 2523-31.
  10. Nosrat Abadi M, Malek-Nia N. Comparative measurements of plasma beta-endorphins in normal subjects and drug addicts. Proceedings of The First Iranian Congress of Biochemistry; 1991; Zahedan, Iran. p. 116.
  11. Petraglia F, Bacchi MA, Comitini G, Scazzina D, Facchinetti F, Fiaschetti D, et al. Plasma beta-endorphin and beta-lipotropin levels increase in well trained athletes after competition and non competitive exercise. J Endocrinol Invest 1990; 13(1): 19-23.
  12. Maisel AS, Harris T, Rearden CA, Michel MC. Beta-adrenergic receptors in lymphocyte subsets after exercise. Alterations in normal individuals and patients with congestive heart failure. Circulation 1990; 82(6): 2003-10.
  13. Yoon JR, Park SC. Exercise intensity-related responses of B-endorphin, ACTH, and cortisol. Korean Iournal of Sport Science 1991; 3: 21-32.
  14. Schwarz L, Kindermann W. Beta-endorphin, adrenocorticotropic hormone, cortisol and catecholamines during aerobic and anaerobic exercise. Eur J Appl Physiol Occup Physiol 1990; 61(3-4): 165-71.
  15. Hargreaves KM, Flores CM, Dionne RA, Mueller GP. The role of pituitary beta-endorphin in mediating corticotropin-releasing factor-induced antinociception. AJP 1990; 258(2): 235-42.
  16. Sutton JR, Vell F, Harber VJ. Hormonal adaptation to physical activity.bouchard, C & Collegueseds.exercise, fitness, and health. Illinois: Human Kinetics Books; 1990.
  17. Farzanaki P, Azarbayjani MA, Rasaee MJ, Jourkesh M, Ostojic SM, Stannard S. Salivary immunoglobulin A and cortisol response to training in young elite femaile gymnasts. Brazilian Journal of Biomotricity 2008; 252-8.
  18. Hindmarsh KW, Tan L, Sankaran K, Laxdal VA. Clinical Investigation Diurnal Rhythms of Cortisol, ACTH, and p-Endorphin Levels in Neonates and Adults. The Western Journal of Medicine 1989; 151(2): 153-6.
  19. Tagashira S, Yamaguchi K, Matsunaga T, Toda K, Hayashi Y. Salivary prekallikrein output during the ranger training-induced stress. Stress and Health 2004; 20(5): 249-53.
Journal of Isfahan Medical School
Volume 29, Issue 166 - Serial Number 166
November and December 2012
Pages 2298-2306

Files

History

  • Receive Date: 15 April 2011
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics