Identification of Capsular Serotype and Antibiotic Resistance of Streptococcus Agalactiae Isolated from Pregnant Women

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Microbiology, School of Medicine AND Reproductive Immunology Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Yazd, Iran

2 Assistant Professor, Department of Laboratory Sciences, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Yazd, Iran

3 Associate Professor, Department of Microbiology, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, Yazd, Iran

4 Associate Professor, Department of Community Medicine, School of Medicine AND Health Monitoring Research Center, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

Abstract

Background: Streptococcus agalactiae (Group B Streptococcus or GBS) is a natural resident of the gastrointestinal tract of healthy individuals, and is the most common cause of sepsis, meningitis, pneumonia, and severe diseases in newborns. Resistance to penicillin and macrolides, as selective antibiotics for the treatment of GBS infections, is increasing. In this study, we aimed to determine the capsule serotypes and antibiotic resistance pattern of Streptococcus agalactiae isolated from pregnant women referred to the Valiasr hospital in Borujen City, Iran.Methods: In this descriptive cross-sectional study, a total of 760 vaginal samples were collected from pregnant women in the period between 35-37 weeks of gestation. Following conventional phenotypic evaluation and GBS confirmation, GBS isolates resistance to antibiotic disc was assessed through disk diffusion test. Finally, capsular serotypes were determined using multiplex polymerase chain reaction (multiplex PCR) method.Findings: Out of 760 pregnant women participating in this study, 70 (9.2%) were carriers of GBS. Our finding disclosed that the lowest and highest resistance among GBS isolates were related to penicillin (0%), and tetracycline (92.85%), respectively. The most common capsular serotypes were III (45.71%), followed by II (17.14%), VI (14.28%), Ib (11.42%), V (8.57%), and Ia (2.85%), respectively.Conclusion: In this study, we detected low prevalence of GBS, as well as low level resistance to macrolides. In addition, predominant capsular serotype was III.

Keywords

References

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History

  • Receive Date: 08 March 2021
  • Revise Date: 02 November 2024
  • Accept Date: 06 April 2022

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