Effects of Aqueous Extract of Trachyspermum Copticum (L.) link Seeds on Transcription of Transforming Growth Factor-beta Receptor 1 and 2 Genes in Cancer Cells

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

2 Associate Professor, Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3 Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 PhD Candidate, Department of Immunology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5 Lecturer, Department of Pharmacognosy, School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, Iran

6 Associate Professor, Department of Community Medicine, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Background: Transforming growth factor (TGF)-beta 2 signaling pathways and their receptors exert a pivotal role in behavior and fate of tumor cells. Therefore, these pathways are target of drug discovery researches. In this study, we evaluated the effects of the aqueous extract of Trachyspermum copticum (L.) link (carum) seeds on TGF-beta receptor 1 and 2 genes coding the heterodimer complex of receptor in MCF-7 cell line of human breast cancer.Methods: Non-cytotoxic concentrations of the extract determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide (MTT) test and cells treated with non-cytotoxic concentrations (5 and 40 µg/ml) of extract for 24 hours. Then, to evaluate the expression of TGF-beta receptors 1 and 2 genes at the level of transcription, real-time polymerase chain reaction (PCR) was performed (comparing the control group). Data were analyzed using REST® software and analysis of variance test. Level of significance was set at 0.05.Findings: Cells treated with concentration of 5 µg/ml of extract showed reduction in gene transcription levels to almost half (0.52) (P = 0.015); and in cells treated with concentration of 40 µg/ml of extract, gene transcription levels increased to 1.34 times (P = 0.066) comparing to control group. Treatment of cells with extract showed no meaningful changes in TGF-beta receptor 2 gene expression at the level of transcription (P = 0.148).Conclusion: Results suggest that treatment of MCF-7 cells with low concentration of aqueous extract of Trachyspermum copticum (L.) link seeds can affect the TGF-beta receptor 1 gene transcription, but not the TGF-beta receptor 2; and alter the microenvironment of tumor cell. Further studies have to be performed for conclusive evidence.

Keywords

References

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Journal of Isfahan Medical School
Volume 35, Issue 437 - Serial Number 437
May and June 2017
Pages 801-806

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History

  • Receive Date: 07 May 2017
  • Revise Date: 02 November 2024
  • Accept Date: 06 April 2022

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