miR-370 Expression Analysis in Breast Cancer

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Immunology, School of Medicine AND Student Research Committee, Isfahan University of Medical sciences, Isfahan, Iran

2 Assistant Professor, Department of Immunology, School of Medicine AND Applied Physiology Research Center, Isfahan University of Medical sciences, Isfahan, Iran

3 Professor, Department of Immunology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran

4 Professor, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 Assistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical sciences, Isfahan, Iran

Abstract

Background: MicroRNAs (MiRNAs) are 21-24 nucleotides which have different levels of expression between tumors and normal tissues. In this study, we analyzed the expression level of miR-370 in different stages and grades of human breast cancer tissues compared with adjacent normal tissues.Methods: In this case-control study, the expression of miR-370 on 22 tissue samples (tumor and normal) from patients with breast cancer was investigated via quantitative real-time polymerase chain reaction method.Findings: miR-370 expression showed a significant increase in tumor tissues compared to normal tissues (P = 0.001) as well as in cancer tissues at stage III (P = 0.004) and grade 3 (P = 0.011) than in other stages.Conclusion: According to evaluation of miR-370 expression in different stages and grades and its relation to more advanced tumor stages/grades, it can possibility act as oncomiRNA in breast cancer. It may be suggested as a prognostic biomarker or therapeutic target in breast cancer, too.

Keywords

References

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Journal of Isfahan Medical School
Volume 33, Issue 339 - Serial Number 339
May and June 2015
Pages 924-931

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History

  • Receive Date: 30 December 2014
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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