Preparation of Fibrin/Poly Vinyl Alcohol Electrospun Nanofibers Scaffold for Tissue Engineering Applications

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 MSc Student, Department of Anatomical Sciences, School of Medicine AND Student research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Associate Professor, Biosensor Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Nowadays, the biodegradable polymer nano-composites have particular importance in tissue engineering because of mechanical properties and good biocompatibility. The aim of this study was to design and evaluate nano-composite fibrin/polyvinyl alcohol (PVA) scaffold using electrospinning method and cell viability on it.Methods: Nano-composite scaffold fibrin/PVA were prepared by electrospinning method while 28.5% of the polymer was formed of fibrin. The porosity of the scaffolds was calculated via scanning electron microscopy by using “Matlab” software and porosity morphology, their distribution and size of the nanofibers. Water absorption test and contact angle measurement were performed. Also, human adipose-derived stem cells were used for cell viability evaluation on scaffolds.Findings: The mean diameter of electrospun fibrin/PVA scaffold was measured 500 nm. The average pore size and porosity of the prepared sample was 1.7 micrometers and 83.81%, respectively. The average contact angle was 31.71 degrees and 24-hour average water absorption was measured 68.5%. Evaluation test of the cell viability has a significant difference compared to control groups.Conclusion: The results of this study show that electrospun scaffolds fibrin/PVA can be used in cartilage and nerve tissue engineering.

Keywords

References

  1. George M, Abraham TE. Polyionic hydrocolloids for the intestinal delivery of protein drugs: alginate and chitosan--a review. J Control Release 2006; 114(1): 1-14.
  2. Rabie A, Esfandiari E, Fesharaki M, Sanaie M, Aminmansur B, Hashemibeni B. Access to a three dimentional osteoblasts culture originating human carvaria in Iran. J Isfahan Med Sch 2010; 27(102): 777-87. [In Persian].
  3. Weisel JW. Fibrinogen and fibrin. Adv Protein Chem 2005; 70: 247-99.
  4. Eyrich D, Brandl F, Appel B, Wiese H, Maier G, Wenzel M, et al. Long-term stable fibrin gels for cartilage engineering. Biomaterials 2007; 28(1): 55-65.
  5. Le Nihouannen D, Guehennec LL, Rouillon T, Pilet P, Bilban M, Layrolle P, et al. Micro-architecture of calcium phosphate granules and fibrin glue composites for bone tissue engineering. Biomaterials 2006; 27(13): 2716-22.
  6. Prasad CK, Muraleedharan CV, Krishnan LK. Bio-mimetic composite matrix that promotes endothelial cell growth for modification of biomaterial surface. J Biomed Mater Res A 2007; 80(3): 644-54.
  7. Sreerekha PR, Krishnan LK. Cultivation of endothelial progenitor cells on fibrin matrix and layering on dacron/polytetrafluoroethylene vascular grafts. Artif Organs 2006; 30(4): 242-9.
  8. Sreerekha PR, Menon D, Nair SV, Chennazhi KP. Fabrication of fibrin based electrospun multiscale composite scaffold for tissue engineering applications. J Biomed Nanotechnol 2013; 9(5): 790-800.
  9. Agarwal S, Wendorff JH, Greiner A. Use of electrospinning technique for biomedical applications. Polymer 2008; 49(26): 5603-21.
  10. Rutledge GC, Fridrikh SV. Formation of fibers by electrospinning. Adv Drug Deliv Rev 2007; 59(14): 1384-91.
  11. Teo WE, Ramakrishna S. A review on electrospinning design and nanofibre assemblies. Nanotechnology 2006; 17(14): R89-R106.
  12. Christman KL, Vardanian AJ, Fang Q, Sievers RE, Fok HH, Lee RJ. Injectable fibrin scaffold improves cell transplant survival, reduces infarct expansion, and induces neovasculature formation in ischemic myocardium. J Am Coll Cardiol 2004; 44(3): 654-60.
  13. Humphrey RG, Smith SD, Pang L, Sadovsky Y, Nelson DM. Fibrin enhances differentiation, but not apoptosis, and limits hypoxic injury of cultured term human trophoblasts. Placenta 2005; 26(6): 491-7.
  14. Ravichandran R, Venugopal JR, Sundarrajan S, Mukherjee S, Sridhar R, Ramakrishna S. Expression of cardiac proteins in neonatal cardiomyocytes on PGS/fibrinogen core/shell substrate for Cardiac tissue engineering. Int J Cardiol 2013; 167(4): 1461-8.
  15. Perumcherry SR, Chennazhi KP, Nair SV, Menon D, Afeesh R. A novel method for the fabrication of fibrin-based electrospun nanofibrous scaffold for tissue-engineering applications. Tissue Eng Part C Methods 2011; 17(11): 1121-30.
  16. Yang SH, Wu CC, Shih TT, Chen PQ, Lin FH. Three-dimensional culture of human nucleus pulposus cells in fibrin clot: comparisons on cellular proliferation and matrix synthesis with cells in alginate. Artif Organs 2008; 32(1): 70-3.
  17. Vallet-Regi M, Romero AM, Ragel CV, LeGeros RZ. XRD, SEM-EDS, and FTIR studies of in vitro growth of an apatite-like layer on sol-gel glasses. J Biomed Mater Res 1999; 44(4): 416-21.
  18. Ghasemi-Mobarakeh L, Semnani D, Morshed M. A novel method for porosity measurement of various surface layers of nanofibers mat using image analysis for tissue engineering applications. J Appl Polym Sci 2007; 106(4): 2536-42.
Journal of Isfahan Medical School
Volume 34, Issue 388 - Serial Number 388
May and June 2016
Pages 737-744

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History

  • Receive Date: 02 March 2016
  • Revise Date: 01 November 2024
  • Accept Date: 06 April 2022

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