Therapeutic Effects of Extracts of Elaeagnus Angustifolia in Experimentally Induced Ulcerative Colitis in Rat

Document Type : Original Article(s)

Authors

1 Professor, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Resident, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Professor, Department of Pharmacology, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

4 Professor, Department of Pharmacognosy, School of Pharmacy, Isfahan University of Medical Sciences, Isfahan, Iran

10.48305/jims.v43.i833.1220

Abstract

Background: Ulcerative colitis is a chronic inflammatory bowel disease (IBD) affecting the rectal and colonic mucosa. Given the anti-inflammatory properties of Elaeagnus angustifolia and its potential effect in the treatment of colitis, this study aimed to determine the effects of the hydroalcoholic extract and chloroform fraction of its fruit in an animal model of induced colitis.
Methods: In this study, 63 male Wistar rats (9 groups of 7 each) were used. Hydroalcoholic extract and chloroform fraction of Elaeagnus angustifolia were administered orally at doses of 100, 200, and 400 mg/kg, two hours before colitis induction and continued for five days. Prednisolone (4 mg/kg) and normal saline (1 ml/kg) were administered orally as positive and negative control groups, respectively. All rats were sacrificed 24 hours after receiving the last dose (day 6), and colon tissue was weighed and examined microscopically, macroscopically, and for myeloperoxidase (MPO) enzyme activity.
Findings: A significant difference was observed between the groups regarding the decrease in distal colon weight. All doses of hydroalcoholic extract and two doses of chloroform fraction (200 and 400 mg/kg) improved macroscopic indices and reduced MPO activity. Two doses of hydroalcoholic extract and chloroform fraction (200 and 400 mg/kg) significantly improved microscopic indices.
Conclusion: Both the hydroalcoholic extract and chloroform fraction of Elaeagnus angustifolia were effective in the treatment of acute induced colitis. With increasing dose, their effectiveness also increased, which is probably due to the biophenols and flavonoids present in the plant.

Highlights

Mozhgan Mokhtari: Google Scholar

Parvin Mahzouni: Google Scholar 

Mohsen Minaiyan: Google Scholar 

Gholamreza Asghari: Google Scholar

Keywords

Main Subjects

References

  1. Jairath V, Feagan BG. Global burden of inflammatory bowel disease. The Lancet Gastroenterology & Hepatology 2020; 5(1): 2-3.
  2. Le Berre C, Ananthakrishnan AN, Danese S, Singh S, Peyrin-Biroulet L. Ulcerative colitis and Crohn’s disease have similar burden and goals for treatment. Clin Gastroenterol Hepatol 2020; 18(1): 14-23.
  3. Chami B, Ahmad G, Schroder A, San Gabriel P, Witting P. The role of myeloperoxidase and neutrophil extracellular traps in the pathogenesis of inflammatory bowel disease. Gastroenterology 2021; 160(3): S5-S6.
  4. Honour JW. Biochemistry of the menopause. Ann Clin Biochem 2018; 55(1): 18-33.
  5. Kaltsa O, Lakka A, Grigorakis S, Karageorgou I, Batra G, Bozinou E, et al. A green extraction process for polyphenols from elderberry (Sambucus nigra) flowers using deep eutectic solvent and ultrasound-assisted pretreatment. Molecules 2020; 25(4): 921.
  6. Kahrizsangi AG, Haidari F, Yousefi L. The effect of eight weeks of water exercise with supplementation Elaeagnus angustifolia on some inflammatory and growth factors in overweight postmenopausal women [in Persian]. J Birjand Univ Med Sci 2022; 29(4): 343-54.
  7. Moezzi N, Najafzadeh Varzi H, Shirali S. Comparing the effect of Elaeagnus angustifolia L. extract and Lowsonia intermis L. paste, with silver sulfadiazine ointment on wound healing in rat [in Persian]. Iranian Journal of Medicinal and Aromatic Plants Research 2009; 25(2): 253-60.
  8. Dehghan MH, Soltani J, Kalantar E, Farnad M, Kamalinejad M, Khodaii Z, et al. Characterization of an Antimicrobial Extract from Elaeagnus angustifolia. Int J Enteric Pathog 2014; 2(3): e20157.
  9. Shabani M, Rezaei A, Badehnoosh B, Qorbani M, Yaseri M, Ramezani R, et al. The effects of Elaeagnus angustifolia L. on lipid and glycaemic profiles and cardiovascular function in menopausal women: A double‐blind, randomized, placebo‐controlled study. Int J Clin Pract 2021; 75(4): e13812.
  10. Taskhiri Z, Mehraban SM, Nazari Z. The effect of oleaster (ElaeagnusAngustifolia) powder on antioxidant, tannin and sensory properties of mint chocolate [in Persian]. JFST 2019; 90(16): 101-12.
  11. Nazir N, Zahoor M, Nisar M. A review on traditional uses and pharmacological importance of genus Elaeagnus species. Botanical Review 2020; 86(3-4): 247-80.
  12. Mamashli M, Nasseri S, Mohammadi Y, Ayati S, Zarban A. Anti-inflammatory effects of N-Acetylcysteine and Elaeagnus angustifolia extract on acute lung injury induced by λ-carrageenan in rat. Inflammopharmacology 2022; 30(5): 1759-68.
  13. Hamidpour R, Hamidpour S, Hamidpour M, Shahlari M, Sohraby M, Shahlari N, et al. Russian olive (Elaeagnus angustifolia L.): From a variety of traditional medicinal applications to its novel roles as active antioxidant, anti-inflammatory, anti-mutagenic and analgesic agent. J Tradit Complement Med 2017; 7(1): 24-29.
  14. Minaiyan M, Asghari G, Taheri D, Saeidi M, Nasr-Esfahani S. Anti-inflammatory effect of Moringa oleifera Lam. seeds on acetic acid-induced acute colitis in rats. Avicenna J Phytomed 2014; 4(2): 127-36.
  15. Heidari B, Sajjadi SE, Minaiyan M. Effect of Coriandrum sativum hydroalcoholic extract and its essential oil on acetic acid-induced acute colitis in rats. Avicenna J Phytomed 2016; 6(2): 205-14.
  16. Motevalian M, Shiri M, Shiri S, Shiri Z, Shiri H. Anti-inflammatory activity of Elaeagnus angustifolia fruit extract on rat paw edema. J Basic Clin Physiol Pharmacol 2017; 28(4): 377-81.
  17. Cooper HS, Murthy S, Shah R, Sedergran D. Clinicopathologic study of dextran sulfate sodium experimental murine colitis. Lab Invest 1993; 69(2): 238-49.
  18. Gupta M, Gulati M, Kapoor B, Kumar B, Kumar R, Kumar R, et al. Anti-ulcerogenic effect of methanolic extract of Elaeagnus conferta Roxb. seeds in Wistar rats. J Ethnopharmacol 2021; 275: 114115.
  19. Abulizi X, Shi M-h, Jia Y-m, Xu L, Shi L-l, Pan L. Elaeagnus angustifolia L. fruit alleviates diarrhea via regulating intestinal microbiota and short chain fatty acids. Heliyon 2024; 10(19): e38646.
  20. Akkol E, Ağören BK, Pınarbaşı ÖS, Turan T. Investigating the anti-inflammatory potential of Elaeagnus an-gustifolia L. through enzyme inhibition assays. PHYTONutrients 2024; 3: 11-22.
  21. Sabouri S, Rad AH, Peighambardoust SH, Fathipour RB, Feshangchi J, Ansari F, et al. The oleaster (Elaeagnus angustifolia): A comprehensive review on its composition, ethnobotanical and prebiotic values. Curr Pharm Biotechnol 2021; 22(3): 367-79.
  22. Pascariu O-E, Israel-Roming F. Bioactive compounds from elderberry: Extraction, health benefits, and food applications. Processes 2022; 10(11): 2288.
  23. Nikniaz Z, Mahdavi R, Nikniaz L. Assessment of the effect of elaeagnus angustifolia on anthropometric characteristics and dietary intake in patients with osteoarthritis: a randomized clinical trial. International Journal of Drug Research in Clinics 2023; 1(1): e20-e.
  24. Sedaghat Talab M, Emadi O, Roostaee N, Azar Mehr N, Bijani F, Dousti Motlaq A. Antioxidative Effect of Hydroalcoholic Extracts of Fruit and Pit of Elaeagnud Angosifolia Against Oxidative Stress Markers in Benign Prostatic Hyperplasia [in Persian]. Armaghan-e-Danesh 2023; 28(2): 205-17.
Journal of Isfahan Medical School
Volume 43, Issue 833
1st Week, December
November and December 2026
Pages 1220-1227

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History

  • Receive Date: 29 December 2024
  • Accept Date: 13 September 2025

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