Local Brain Ischemia Inducing in Rat by Embolic Model of Stroke

Document Type : Original Article(s)

Authors

1 Assistant Professor, Department of Physiology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

2 MSc, Department of Physiology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran

3 Assistant Professor, Department of Physiology, School of Medicine, Urmia University of Medical Sciences, Urmia, Iran

Abstract

AbstractIn patients affected with stroke, most ischemic episodes (80-90%) occur due to occlusion of middle cerebral artery (MCA).Background:Some of blood through femoral artery was withdrawn into a PE- 50 catheter and kept for 2 h in room temperature and 22 h at 4°C to allow old clot formation. Then the catheter was advanced 17 mm in the internal carotid artery until its tip was 1 to 2 mm away from MCA origin. 3-5 µl the preformed clot or 5 µl saline (for sham- operated animals) were injected. Behavioral deficits and seizure activities were recorded at 2, 24, and 48 hours after clot injection. Then the rats were decapitated to remove the brains and prepare them for Tetrazoliume chloride (TTC) staining and analyzing.Methods:When 3 or 5 µl clots were injected, infarct volume was 29.35±1.26% and 16.15±94% respectively (p<0.05). Brain edema also was 8.6±1.65% and 3±1.74%, respectively (p<0.05). There was a significant correlation between infarct volume and brain edema (r=0.56, p<0.05). Behavioral deficit score at 48 hours after clot injection between two groups were significantly different (p<0.05).Findings:This model is very similar to thromboembolic stroke in human and prepares a reliable method for investigating the stroke mechanism. It would also be useful for studying thromboembolic agents' effect on stroke and brain ischemic injury.Conclusion:Brain ischemia, embolic model, brain edema, neurological deficits.Key words:

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History

  • Receive Date: 07 November 2010
  • Revise Date: 30 April 2024
  • Accept Date: 06 April 2022

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