Journal of Isfahan Medical School

Journal of Isfahan Medical School

Corelation of Homologous Recombination Repair System by Studying a Single-nucleotide Polymorphism in Xrcc3 Gene with Initiation and Progression of Colorectal Cancer

Document Type : Original Article (s)

Authors
Majid Motovali-Bashi 1
Jafar Maleki 2
Simin Hemmati 3
Hassan Korbekandi 4
1 Assistant Professor, Division of Genetics, Department of Biology, School of Sciences, The University of Isfahan, Isfahan, Iran.
2 Department of Biology, School of Sciences, The University of Isfahan, Isfahan, Iran.
3 Assistant Professor, Department of Oncology and Radiotherapy, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
4 Assistant Professor, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Abstract
Background: Colorectal cancer is the third cause of cancer death in western countries. Age, inadequate diet, obesity, inactivity and genetic changes are some of the risk factors of colorectal cancer. Corelation of genetic diversity in homologous recombination repair system with cancer was evaluated in many recent studies. This study was done to investigate the correlation of T241M polymorphism in Xrcc3 gene and colorectal cancers.Methods: In this cross-sectional study after collecting blood samples and extraction genomic DNA, genotype distribution of the polymorphism was determined by RFLP-PCR (Restriction fragment lengh-polymerase-Polymerase chain reaction) method.Finding: A significant corelation between T241M polymorphism with colorectal cancer was seen. Age and family history were also corelated with this cancer. Although, there was no statistically relationship between smoking status and colon cancer, but it showed correlation with rectum cancer and it has been also observed that the most occurance of metastatic activity is in the rectum. Conclusion: According to our study T241M polymorphism in Xrcc3 gene from homologous recombination repair systm could be a suitable factor for early diagnosis of colorectal cancer especially rectum and its co-operation with smoking status. 
Keywords
Colorectal Cancer
Homologous recombination repair system
Xrcc3 gene
RFLP-PCR
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Journal of Isfahan Medical School
Volume 29, Issue 137 - Serial Number 137
March and April 2011
Pages 518-525

  • Receive Date 10 June 2011
  • Accept Date 06 April 2022