Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effects of L-Arginine (the Precursor of Nitric Oxide) and L-NAME (Nitric Oxide Synthase Inhibitor) on Coronary Angiogenesis in Normal Rats

Document Type : Original Article (s)

Authors
Majid Khazaei 1
Mohammad Amin Moshayedi 2
Massoud Teimouri 2
Shahrzad Aghili 2
Saeed Montazeri 2
Roshanak Mehdipour 2
Fazolah Hashemzehi 2
Hourossadat Hashemi Jazi 2
1 Associate Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Student of Medicine, School of Medicine And Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Nitric oxide (NO) has beneficial effects on cardiovascular system including improvement of angiogenesis. The aim of this study was to evaluate the effects of L-arginine (the precursor of NO) and L-NG-nitroarginine methyl ester (L-NAME) (NO synthase inhibitor) on serum vascular endothelial growth factor (VEGF) and coronary angiogenesis in normal rats.Methods: In this study, 18 male rats were randomly divided into 3 groups. Groups 1 and 2 received intraperitoneal L-NAME (10 mg/kg/day) and intraperitoneal L-arginine (50 mg/kg/day), respectively. Group 3 (control) received normal saline with the same volume. After 3 weeks, blood samples were taken for measuring serum VEGF and NO concentrations. Coronary angiogenesis was also evaluated using immunohistochemistry method.Findings: Serum NO concentration in the control group was 6.45 ± 0.44 µmol/l. Serum NO concentrations were increased by administration of L-arginine (7.90 ± 0.75 vs. 6.45 ± 0.44 µmol/l) and decreased by administration of L-NAME (4.86 ± 0.40 vs. 6.45 ± 0.44 µmol/l). L-arginine significantly increased serum VEGF concentration (353.01 ± 7.03 vs. 100.50 ± 6.61 pg/ml; P < 0.05). L-arginine and L-NAME could not change capillary density in heart tissue. Conclusion: Although L-arginine could change some serum angiogenic factors, neither L-arginine nor L-NAME could alter coronary angiogenesis in normal rats.
Keywords
L-arginine
L-NAME
Nitric oxide
Vascular endothelial growth factor
Angiogenesis
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Journal of Isfahan Medical School
Volume 29, Issue 158 - Serial Number 158
September and October 2011
Pages 1630-1636

  • Receive Date 24 December 2011
  • Accept Date 06 April 2022