Document Type : Original Article (s)
Authors
1
Assistant Professor, Department of Pharmacology and Toxicology AND Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
2
Professor, Department of Pharmacognosy AND Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
3
Professor, Department of Pharmacology and Toxicology AND Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran
4
PhD Student, Department of Pharmacology AND Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Kelussia odoratissima Mozaffarian is an endemic plant which grows wildly in Iran. The plant is used as food supplement and medical herb in traditional medicine. Extracts and essential oil of the plant have been proved to possess anti-inflammatory, sedative, fibrinolytic, antacid, memory enhancer and antioxidant effects. Damage to deoxyribonucleic acid (DNA) has a very important role in mutagenesis, aging, and cancer. This study aimed to evaluate the protective effects of methanolic and aqueous extracts of K. odoratissima on DNA damage induced by methyl methanesulfonate (MMS). Methods: The comet assay method was selected to evaluate the antigenotoxicity as it is a fast, sensitive, inexpensive, and easy method to perform. Therefore, after incubation of different concentrations of extracts with liver hepatocellular cells (HepG2) for one hour, cells were exposed to MMS for an hour. Then, cell suspension was added to precoated normal agarose slides. After lysing the cells with lysis solution, electrophoresis method was used to assess DNA damage. Using comet score, tail length, percent of DNA in tail, and tail moment were measured. Findings: Concentrations of 5, 10, 50 µg/ml of aqueous extracts and 0.5, 1, 5 µg/ml of methanolic extracts of K. odoratissima significantly reduced MMS-induced damage to DNA of HepG2 cells. Conclusion: We observed the antigenotoxic or genoprotective effect of aqueous and methanolic extracts of K. odoratissima against DNA damage caused by MMS. Therefore, the use of K. odoratissima can probably prevent the complications of these damages. Keywords: Comet assay, Kelussia odoratissima Mozaffarian, Genoprotective, Antigenotoxicity
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