Genotyping of Salmonella Enterica Serovar Enteritidis Strains Isolated from Clinical Samples by Pulsed-Field Gel Electrophoresis (PFGE)

Document Type : Original Article (s)

Authors

1 Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Associate Professor, Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran

Abstract

Background: The infections caused by Salmonella enterica serovar enteritidis (Salmonella enteritidis) have been increasing in developed and developing countries. The aim of this study was to investigate the genetic relationship between the strains of Salmonella enteritidis.Methods: In a descriptive study from 2008 to 2010, clinical Salmonella enteritidis strains were collected from the patients referred to different hospitals in Tehran. The strains identified by biochemical and serological methods. Genetic relationships between the isolates were investigated using pulsed field gel electrophoresis (PFGE) method.Findings: Of 40 Salmonella enteritidis isolated strains, two PFGE profiles were obtained which among them, 32 (80%) and 8 (20%) of isolates belonged to cluster A and cluster B, respectively. Based on PFGE patterns, 28 (70%) strains exhibited nine bands and were belonged to cluster A.Conclusion: The result of this study indicated that isolated Salmonella enteritidis strains belonged to the closed clones.

Keywords

References

  1. Ruby T, McLaughlin L, Gopinath S, Monack D. Salmonella's long-term relationship with its host. FEMS Microbiol Rev 2012; 36(3): 600-15.
  2. Yue M, Rankin SC, Blanchet RT, Nulton JD, Edwards RA, Schifferli DM. Diversification of the Salmonella fimbriae: a model of macro- and microevolution. PLoS One 2012; 7(6): e38596.
  3. Ranjbar R, Sarshar M. Genetic diversity of clinical strains of Salmonella enterica serovar Typhimurium. J Mil Med 2012; 14(2): 143-7.
  4. Wain J, Hosoglu S. The laboratory diagnosis of enteric fever. J Infect Dev Ctries 2008; 2(6): 421-5.
  5. Lopez FE, de las Mercedes Pescaretti M, Morero R, Delgado MA. Salmonella Typhimurium general virulence factors: a battle of David against Goliath? Food Research International 2012; 45(2): 842-51.
  6. Ranjbar R, Giammanco GM, Farshad S, Owlia P, Aleo A, Mammina C. Serotypes, antibiotic resistance, and class 1 integrons in Salmonella isolates from pediatric cases of enteritis in Tehran, Iran. Foodborne Pathog Dis 2011; 8(4): 547-53.
  7. Ranjbar R, Giammanco GM, Aleo A, Plano MR, Naghoni A, Owlia P, et al. Characterization of the first extended-spectrum beta-lactamase-producing nontyphoidal Salmonella strains isolated in Tehran, Iran. Foodborne Pathog Dis 2010; 7(1): 91-5.
  8. Pang JC, Chiu TH, Helmuth R, Schroeter A, Guerra B, Tsen HY. A pulsed field gel electrophoresis (PFGE) study that suggests a major world-wide clone of Salmonella enterica serovar Enteritidis. Int J Food Microbiol 2007; 116(3): 305-12.
  9. Sarshar M, Ranjbar R, Shahrokhi N, Sadeghifard N, Hassani A, Nasrabadi Z. Detection of Escherichia coli, Salmonella enterica and Shigella dysenteriae by analysis of 23S ribosomal DNA gene. J Isfahan Med Sch 2013; 30(219): 2333-43.
  10. Tsen HY, Lin JS, Hsih HY. Pulsed field gel electrophoresis for animal Salmonella enterica serovar Typhimurium isolates in Taiwan. Vet Microbiol 2002; 87(1): 73-80.
  11. Ranjbar R, Sarshar M, Morovvati S. A study of ribotype patterns of salmonella enterica serovar enteritidis strains isolated in Tehran, Iran. J Isfahan Med Sch 2012; 30(180): 238-47.
  12. Amini K, Zahraei Salehi T, Nikbakht Gh, Ranjbar R, Amini J, Ashrafganjooei ShB. Molecular detection of invA and spv virulence genes in Salmonella enteritidis isolated from human and animals in Iran. Afr J Microbiol Ref 2010; 4(21): 2202-10.
  13. Cesare A, Manfreda G. Use of the automated ribotyping for epidemiological Investigations. Ann Microbiol 2002; 52: 181-90.
  14. Tenover FC. Plasmid fingerprinting. A tool for bacterial strain identification and surveillance of nosocomial and community-acquired infections. Clin Lab Med 1985; 5(3): 413-36.
  15. Landers E, Gonzalez-Hevia MA, Mendoza MC. Molecular epidemiology of Salmonella serotype Enteritidis. Relationships between food, water and pathogenic strains. Int J Food Microbiol 1998; 43(1-2): 81-90.
  16. de Oliveira FA, Geimba MP, Pasqualotto AP, Brandelli A, Pasquali G, da Silva WP, et al. Clonal relationship among Salmonella enterica serovar Enteritidis involved in foodborne outbreaks in Southern Brazil. Food Control 2009; 20(6): 606-10.
  17. Ranjbar R, Sarshar M, Sadeghifard N. Characterization of genetic diversity among clinical strains of Salmonella enterica serovar infantis by ribotyping method. J Zanjan Uni Med Sci 2012; 20(81): 75-84. [In Persian].
  18. Thong KL, Ngeow YF, Altwegg M, Navaratnam P, Pang T. Molecular analysis of Salmonella enteritidis by pulsed-field gel electrophoresis and ribotyping. J Clin Microbiol 1995; 33(5): 1070-4.
  19. Ridley AM, Threlfall EJ, Rowe B. Genotypic characterization of Salmonella enteritidis phage types by plasmid analysis, ribotyping, and pulsed-field gel electrophoresis. J Clin Microbiol 1998; 36(8): 2314-21.
  20. Grimont F, Grimont PA. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol 1986; 137B(2): 165-75.
  21. Ranjbar R, Mirzaee A. Determining of the variety of genotypes in Salmonella typhimurium by ERIC-PCR. J Babol Univ Med Sci 2013: 15(1): 51-7. [In Persian].
  22. Stull TL, LiPuma JJ, Edlind TD. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis 1988; 157(2): 280-6.
  23. Leekitcharoenphon P, Lukjancenko O, Friis C, Aarestrup FM, Ussery DW. Genomic variation in Salmonella enterica core genes for epidemiological typing. BMC Genomics 2012; 13: 88.
  24. Stepan RM, Sherwood JS, Petermann SR, Logue CM. Molecular and comparative analysis of Salmonella enterica Senftenberg from humans and animals using PFGE, MLST and NARMS. BMC Microbiol 2011; 11: 153.
  25. Noda T, Murakami K, Asai T, Etoh Y, Ishihara T, Kuroki T, et al. Multi-locus sequence typing of Salmonella enterica subsp. enterica serovar Enteritidis strains in Japan between 1973 and 2004. Acta Vet Scand 2011; 53: 38.
  26. Pang JC, Chiu TH, Chiou CS, Schroeter A, Guerra B, Helmuth R, et al. Pulsed-field gel electrophoresis, plasmid profiles and phage types for the human isolates of Salmonella enterica serovar Enteritidis obtained over 13 years in Taiwan. J Appl Microbiol 2005; 99(6): 1472-83.
  27. Swaminathan B, Barrett TJ, Hunter SB, Tauxe RV. PulseNet: the molecular subtyping network for foodborne bacterial disease surveillance, United States. Emerg Infect Dis 2001; 7(3): 382-9.
  28. Tsen HY, Lin JS. Analysis of Salmonella enteritidis strains isolated from food-poisoning cases in Taiwan by pulsed field gel electrophoresis, plasmid profile and phage typing. J Appl Microbiol 2001; 91(1): 72-9.
  29. Rivoal K, Protais J, Queguiner S, Boscher E, Chidaine B, Rose V, et al. Use of pulsed-field gel electrophoresis to characterize the heterogeneity and clonality of Salmonella serotype Enteritidis, Typhimurium and Infantis isolates obtained from whole liquid eggs. Int J Food Microbiol 2009; 129(2): 180-6.
  30. Fromin N, Hamelin J, Tarnawski S, Roesti D, Jourdain-Miserez K, Forestier N, et al. Statistical analysis of denaturing gel electrophoresis (DGE) fingerprinting patterns. Environ Microbiol 2002; 4(11): 634-43.
  31. Adesiyun A, Carson A, McAdoo K, Bailey C. Molecular analysis of Salmonella enteritidis isolates from the Caribbean by pulsed-field gel electrophoresis. Rev Panam Salud Publica 2000; 8(5): 342-7.
  32. Ranjbar R, Salimkhani E, Sadeghifard N, Yazdi JZ, Morovvati S, Jonaidi N, et al. An outbreak of gastroenteritis of unknown origin in Tehran, July 2003. Pak J Biol Sci 2007; 10(7): 1138-40.
  33. Farahani N, Mirnejad R, Ahmadi Z, Amirmozafari N, Masjedian F. Molecular Typing of Acinetobacter baumannii Clinical Strains in Tehran by Pulsed-Field Gel Electrophoresis. J Fasa Univ Med Sci 2013; 2(4): 259-65. [In Persian].
  34. Olsen JE, Skov MN, Threlfall EJ, Brown DJ. Clonal lines of Salmonella enterica serotype Enteritidis documented by IS200-, ribo-, pulsed-field gel electrophoresis and RFLP typing. J Med Microbiol 1994; 40(1): 15-22.
  35. Khakabimamaghani S, Najafi A, Ranjbar R, Raam M. GelClust: A software tool for gel electrophoresis images analysis and dendrogram generation. Comput Methods Programs Biomed 2013; 111(2): 512-8.
  36. Ben-Darif E, Jury F, De PE, Threlfall EJ, Bolton FJ, Fox AJ, et al. Development of a multiplex primer extension assay for rapid detection of Salmonella isolates of diverse serotypes. J Clin Microbiol 2010; 48(4): 1055-60.
  37. Naghoni A, Ranjbar R, Tabaraie B, Farshad S, Owlia P, Safiri Z, et al. High prevalence of integron-mediated resistance in clinical isolates of Salmonella enterica. Jpn J Infect Dis 2010; 63(6): 417-21.
  38. Anvarinejad M, Farshad Sh, Hoseini M. Comparison of genetic patterns of E. coli strains isolated from patients with cystitis and pyelonephritis, using pulsed field gel electrophoresis. J Kerman Univ Med Sci 2011; 18(3): 207-17. [In Persian].
  39. Anvarinejad M, Farshad Sh, Alborzi A, Ranjbar R, Hoseini M. Pulsed field gel electrophoresis protocol for typing of uropathogenic Escherichia coli. J Jahrom Univ Med Sci 2011; 9(2): 1-7 [In Persian].
  40. Zou W, Lin WJ, Foley SL, Chen CH, Nayak R, Chen JJ. Evaluation of pulsed-field gel electrophoresis profiles for identification of Salmonella serotypes. J Clin Microbiol 2010; 48(9): 3122-6.
  41. Murase T, Okitsu T, Suzuki R, Morozumi H, Matsushima A, Nakamura A, et al. Evaluation of DNA fingerprinting by PFGE as an epidemiologic tool for Salmonella infections. Microbiol Immunol 1995; 39(9): 673-6.
  42. Campioni F, Moratto Bergamini AM, Falcao JP. Genetic diversity, virulence genes and antimicrobial resistance of Salmonella Enteritidis isolated from food and humans over a 24-year period in Brazil. Food Microbiol 2012; 32(2): 254-64.
Journal of Isfahan Medical School
Volume 31, Issue 240 - Serial Number 240
May and June 2013
Pages 819-829

Files

History

  • Receive Date: 28 January 2013
  • Revise Date: 07 May 2024
  • Accept Date: 06 April 2022

Share

How to cite

Statistics