Journal of Isfahan Medical School

Journal of Isfahan Medical School

Effects of Zinc Protoporphyrin (Znpp) with Radiotherapy on Melanoma Tumor Growth in C57BL6 Mice

Document Type : Original Article (s)

Authors
Amir-Abbas Samety 1
Faezeh Mortazavi 2
Khatereh Abdolmaleki 2
Atieh Hashemian 1
Shaghayegh Haghjooy-Javanmard 3
1 Student of Dentistry, Dental Students' Research Committee, School of Dentistry, Isfahan University of Medical Sciences, Isfahan, Iran
2 Student of Medicine, Medical Students' Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department of Physiology, School of Medicine AND Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Decrease in Heme Oxygenase-1 gene expression is associated with fewer amounts of reoccurrence and better final response to treatment in some cancers. The aim of this study was to analyze the effect of Zinc Protoporphyrin (Znpp), as Heme Oxygenase-1 (HO-1) inhibitor, and radiotherapy on melanoma cells in mice.Methods: In this experimental study, 24 mice were randomly divided to 4 groups. On the first day of the study, melanoma cells were injected and the mice were treated for 16 days. First and second groups received 2000 mg/kg of Znpp every other day. Third and forth groups were injected diluted liquid of Znpp. The first and third groups received 12 gray of radiotherapy on 8th day. The mice were examined each day for any palpable tumors and size was measured. The tumors were extracted on the 16th day. The effect of HO-1 on growth of cancer cells was studied using mitotic index and the size of tumors were measured on 8th and 16th days.Findings: Size of tumor and mitotic index in mice treated with Znpp and radiotherapy was significantly smaller than in control groups (P < 0.05). Size of tumor in mice treated by radiotherapy and Znpp was less than group only treated with radiotherapy (P < 0.05).Conclusion: The HO-1 inhibitor with radiotherapy may have therapeutic effects and it may be more effective in reducing the tumor’s growth than using radiotherapy alone.
Keywords
Melanoma
Heme oxygenase-1
Mitotic index
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Journal of Isfahan Medical School
Volume 31, Issue 263 - Serial Number 263
November and December 2014
Pages 1965-1972

  • Receive Date 27 April 2013
  • Accept Date 06 April 2022