The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients

Document Type : Original Article (s)

Authors

1 Department of Cell and Molecular Biology, School of Basic Sciences and Technologies, University of Science and Culture, Tehran, Iran

2 Associate Professor, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran

3 Associate Professor, Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran

4 Associate Professor, Department of Genetics, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran

5 Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), Academic Center for Education, Culture and Research (ACECR), Tehran, Iran

Abstract

Background: Infection with hepatitis B virus (HBV) may result in a number of different clinical outcomes. There are strong evidences in HBV infection that host genetic factors play a major role in determining the outcome of infection. Polymorphisms in the promoter region of interleukin 10 (IL-10) affect its production and confer susceptibility to inflammatory diseases. The aim of present study was to investigate the association between HBV infection and -592 polymorphism in the promoter region of the IL-10 gene in Iranian population.Methods: 100 HBV infected patients and 100 healthy individuals were randomly selected. Genomic DNA was extracted from blood buffy coat using the salting-out method. The IL-10-592(A/C) genotypes were determined using allele-specific polymerase chain reaction (PCR) method. The PCR products were electrophoresed on 1.5% agarose gel. The data were analyzed using chi-square test.Findings: The frequency of IL-10-592 C/C genotype was 16% in patients infected with HBV and 12% in healthy individuals and was not significantly different (P = 0.5). Also, the frequency of C variant allele was 55.5% in HBV infected patients and 53% in healthy individuals that was not statistically different (P = 0.6).Conclusion: Based on our findings, there was not any relationship between IL-10-592 (A/C) polymorphism and susceptibility to HBV infection in our study population.

Keywords

References

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Journal of Isfahan Medical School
Volume 31, Issue 271 - Serial Number 271
November and December 2014
Pages 2434-2441

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History

  • Receive Date: 14 September 2013
  • Revise Date: 26 April 2024
  • Accept Date: 06 April 2022

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