1
Department of Cell and Molecular Biology, School of Basic Sciences and Technologies, University of Science and Culture, Tehran, Iran
2
Associate Professor, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3
Associate Professor, Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
4
Associate Professor, Department of Genetics, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
5
Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
Abstract
Background: Infection with hepatitis B virus (HBV) may result in a number of different clinical outcomes. There are strong evidences in HBV infection that host genetic factors play a major role in determining the outcome of infection. Polymorphisms in the promoter region of interleukin 10 (IL-10) affect its production and confer susceptibility to inflammatory diseases. The aim of present study was to investigate the association between HBV infection and -592 polymorphism in the promoter region of the IL-10 gene in Iranian population.Methods: 100 HBV infected patients and 100 healthy individuals were randomly selected. Genomic DNA was extracted from blood buffy coat using the salting-out method. The IL-10-592(A/C) genotypes were determined using allele-specific polymerase chain reaction (PCR) method. The PCR products were electrophoresed on 1.5% agarose gel. The data were analyzed using chi-square test.Findings: The frequency of IL-10-592 C/C genotype was 16% in patients infected with HBV and 12% in healthy individuals and was not significantly different (P = 0.5). Also, the frequency of C variant allele was 55.5% in HBV infected patients and 53% in healthy individuals that was not statistically different (P = 0.6).Conclusion: Based on our findings, there was not any relationship between IL-10-592 (A/C) polymorphism and susceptibility to HBV infection in our study population.
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Bagheri-Mansoori,M H , Sharifi,Z , Sanati,M H , Shahzadeh-Fazeli,A and Farhangnia,M . (2014). The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients. Journal of Isfahan Medical School, 31(271), 2434-2441.
MLA
Bagheri-Mansoori,M H , , Sharifi,Z , , Sanati,M H , , Shahzadeh-Fazeli,A , and Farhangnia,M . "The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients", Journal of Isfahan Medical School, 31, 271, 2014, 2434-2441.
HARVARD
Bagheri-Mansoori M H, Sharifi Z, Sanati M H, Shahzadeh-Fazeli A, Farhangnia M. (2014). 'The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients', Journal of Isfahan Medical School, 31(271), pp. 2434-2441.
CHICAGO
M H Bagheri-Mansoori, Z Sharifi, M H Sanati, A Shahzadeh-Fazeli and M Farhangnia, "The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients," Journal of Isfahan Medical School, 31 271 (2014): 2434-2441,
VANCOUVER
Bagheri-Mansoori M H, Sharifi Z, Sanati M H, Shahzadeh-Fazeli A, Farhangnia M. The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients. JIMS. 2014;31(271):2434-2441 (In Persian).
