Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Survey on -592 Polymorphism of Interlukin-10 in Hepatitis B Virus Infected Patients

Document Type : Original Article (s)

Authors
Mohammad Hassan Bagheri-Mansoori 1
Zohreh Sharifi 2
Mohammad Hossein Sanati 3
Abolhassan Shahzadeh-Fazeli 4
Mansoureh Farhangnia 5
1 Department of Cell and Molecular Biology, School of Basic Sciences and Technologies, University of Science and Culture, Tehran, Iran
2 Associate Professor, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
3 Associate Professor, Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
4 Associate Professor, Department of Genetics, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
5 Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
Abstract
Background: Infection with hepatitis B virus (HBV) may result in a number of different clinical outcomes. There are strong evidences in HBV infection that host genetic factors play a major role in determining the outcome of infection. Polymorphisms in the promoter region of interleukin 10 (IL-10) affect its production and confer susceptibility to inflammatory diseases. The aim of present study was to investigate the association between HBV infection and -592 polymorphism in the promoter region of the IL-10 gene in Iranian population.Methods: 100 HBV infected patients and 100 healthy individuals were randomly selected. Genomic DNA was extracted from blood buffy coat using the salting-out method. The IL-10-592(A/C) genotypes were determined using allele-specific polymerase chain reaction (PCR) method. The PCR products were electrophoresed on 1.5% agarose gel. The data were analyzed using chi-square test.Findings: The frequency of IL-10-592 C/C genotype was 16% in patients infected with HBV and 12% in healthy individuals and was not significantly different (P = 0.5). Also, the frequency of C variant allele was 55.5% in HBV infected patients and 53% in healthy individuals that was not statistically different (P = 0.6).Conclusion: Based on our findings, there was not any relationship between IL-10-592 (A/C) polymorphism and susceptibility to HBV infection in our study population.
Keywords
Single nucleotide polymorphisms (SNPs)
Interleukin-10 (IL-10)
Hepatitis B virus (HBV)
Allele-specific polymerase chain reaction
  1. Lee WM. Hepatitis B virus infection. N Engl J Med 1997; 337(24): 1733-45.
  2. Lavanchy D. Hepatitis B virus epidemiology, disease burden, treatment, and current and emerging prevention and control measures. J Viral Hepat 2004; 11(2): 97-107.
  3. Hohler T, Gerken G, Notghi A, Lubjuhn R, Taheri H, Protzer U, et al. HLA-DRB1*1301 and *1302 protect against chronic hepatitis B. J Hepatol 1997; 26(3): 503-7.
  4. Thursz MR, Kwiatkowski D, Allsopp CE, Greenwood BM, Thomas HC, Hill AV. Association between an MHC class II allele and clearance of hepatitis B virus in the Gambia. N Engl J Med 1995; 332(16): 1065-9.
  5. Guidotti LG, Ando K, Hobbs MV, Ishikawa T, Runkel L, Schreiber RD, et al. Cytotoxic T lymphocytes inhibit hepatitis B virus gene expression by a noncytolytic mechanism in transgenic mice. Proc Natl Acad Sci U S A 1994; 91(9): 3764-8.
  6. Rico MA, Quiroga JA, Subira D, Castanon S, Esteban JM, Pardo M, et al. Hepatitis B virus-specific T-cell proliferation and cytokine secretion in chronic hepatitis B e antibody-positive patients treated with ribavirin and interferon alpha. Hepatology 2001; 33(1): 295-300.
  7. Cheong JY, Cho SW, Hwang IL, Yoon SK, Lee JH, Park CS, et al. Association between chronic hepatitis B virus infection and interleukin-10, tumor necrosis factor-alpha gene promoter polymorphisms. J Gastroenterol Hepatol 2006; 21(7): 1163-9.
  8. Redpath S, Ghazal P, Gascoigne NR. Hijacking and exploitation of IL-10 by intracellular pathogens. Trends Microbiol 2001; 9(2): 86-92.
  9. Moore KW, de Waal MR, Coffman RL, O'Garra A. Interleukin-10 and the interleukin-10 receptor. Annu Rev Immunol 2001; 19: 683-765.
  10. Bidwell J, Keen L, Gallagher G, Kimberly R, Huizinga T, McDermott MF, et al. Cytokine gene polymorphism in human disease: on-line databases. Genes Immun 1999; 1(1): 3-19.
  11. Hohler T, Reuss E, Freitag CM, Schneider PM. A functional polymorphism in the IL-10 promoter influences the response after vaccination with HBsAg and hepatitis A. Hepatology 2005; 42(1): 72-6.
  12. Shin HD, Park BL, Kim LH, Jung JH, Kim JY, Yoon JH, et al. Interleukin 10 haplotype associated with increased risk of hepatocellular carcinoma. Hum Mol Genet 2003; 12(8): 901-6.
  13. Miyazoe S, Hamasaki K, Nakata K, Kajiya Y, Kitajima K, Nakao K, et al. Influence of interleukin-10 gene promoter polymorphisms on disease progression in patients chronically infected with hepatitis B virus. Am J Gastroenterol 2002; 97(8): 2086-92.
  14. Miller SA, Dykes DD, Polesky HF. A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 1988; 16(3): 1215.
  15. Ahn SH, Han KH, Park JY, Lee CK, Kang SW, Chon CY, et al. Association between hepatitis B virus infection and HLA-DR type in Korea. Hepatology 2000; 31(6): 1371-3.
  16. Yang G, Liu J, Han S, Xie H, Du R, Yan Y, et al. Association between hepatitis B virus infection and HLA-DRB1 genotyping in Shaanxi Han patients in northwestern China. Tissue Antigens 2007; 69(2): 170-5.
  17. Yee LJ, Tang J, Gibson AW, Kimberly R, Van Leeuwen DJ, Kaslow RA. Interleukin 10 polymorphisms as predictors of sustained response in antiviral therapy for chronic hepatitis C infection. Hepatology 2001; 33(3): 708-12.
  18. Yan Z, Tan W, Zhao W, Dan Y, Wang X, Mao Q, et al. Regulatory polymorphisms in the IL-10 gene promoter and HBV-related acute liver failure in the Chinese population. J Viral Hepat 2009; 16(11): 775-83.
  19. Turner DM, Williams DM, Sankaran D, Lazarus M, Sinnott PJ, Hutchinson IV. An investigation of polymorphism in the interleukin-10 gene promoter. Eur J Immunogenet 1997; 24(1): 1-8.
  20. Wang C, Zhang X, Zhu B, Hu D, Wu J, Yu R, et al. Relationships between tumour necrosis factor-alpha, interleukin-12B and interleukin-10 gene polymorphisms and hepatitis B in Chinese Han haemodialysis patients. Nephrology (Carlton ) 2012; 17(2): 167-74.
  21. Gao QJ, Liu DW, Zhang SY, Jia M, Wang LM, Wu LH, et al. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection. World J Gastroenterol 2009; 15(44): 5610-9.
Journal of Isfahan Medical School
Volume 31, Issue 271 - Serial Number 271
November and December 2014
Pages 2434-2441

  • Receive Date 14 September 2013
  • Accept Date 06 April 2022