Journal of Isfahan Medical School

Journal of Isfahan Medical School

Isolation, Antibiotic Resistance Determination and Genotypic Analysis of Pseudomonas Aeruginosa Strains Causing Urinary Tract Infection in Catheterized Patients of Shohada and Labafi Nejad Hospitals, Tehran, Iran

Document Type : Original Article (s)

Authors
Nastaran Asghari-Moghadam 1
Reza Rasoolzadeh 2
Seyyed Mohamad-Mahdi Hoseini-Moghadam 3
Mahnaz Seifi 4
Mohamad Reza Pour-Shafie 5
Maliheh Talebi 6
1 Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
2 PhD Student, Department of Biology, Islamic Azad University, Science and Research Branch, Tehran, Iran
3 Associate Professor, Urology and Nephrology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
4 Assistant Professor, Department of Tuberculosis, Pasteur Institute of Iran, Tehran, Iran
5 Professor, Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
6 Assistant Professor, Department of Microbiology, Tehran University of Medical Science, Tehran, Iran
Abstract
Background: Urinary tract infection is the most common nosocomial infection worldwide. Microorganisms causing urinary tract infections are resistant to most of the antibiotics. Pseudomonas aeruginosa (P. aeruginosa) is one of the major causes of nosocomial infections and its antibiotic resistance leads to medical implications associated with urinary tract infections. The aim of this study was to assess the prevalence of P. aeruginosa strains to identify their in-vitro susceptibility to antimicrobial agents and to determine genetic diversity of them.Methods: Urine samples were obtained from catheterized patients in Shohada and Labafi Nejad hospitals, Tehran, Iran, and cultured by standard loop method. The cultures with more than 105 CFU/ml were assumed as positive. After identification of bacterial species by biochemical tests, susceptibility of each isolate was assessed by disk diffusion method according to Clinical and Laboratory Standards Institute (CLSI) guidelines. In order to analyze bacterial genotypic diversity, pulsed-field gel electrophoresis (PFGE) was performed.Findings: 116 out of 163 urine samples were positive for bacterial isolates. Pseudomonas species were placed in third step of the most frequent isolates. Antibiotic sensitivity was observed against most of antimicrobial agents. Some of strains were genetically similar.Conclusion: This study revealed that isolated strains were sensitive to wide range of antibiotic agents. In addition, common type strains were responsible of causing inter- and intra-hospital urinary tract infections in catheterized patients.
Keywords
Urinary tract infection
Catheterization
Pseudomonas aeruginosa
Pulsed-field gel electrophoresis (PFGE)
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Journal of Isfahan Medical School
Volume 32, Issue 272 - Serial Number 272
January and February 2014
Pages 1-8

  • Receive Date 09 June 2013
  • Accept Date 06 April 2022