Journal of Isfahan Medical School

Journal of Isfahan Medical School

Pre-treatment Effects of Eriobotrya Japonica Extraction on Malondialdehyde (MDA), Brain-Derived Neurotrophic Factor (BDNF), and Superoxide Dismutase (SOD) Levels in Hippocampus of Parkinsonian Rats Induced by 6-hydroxydopamine Following 12 weeks of Volunt

Document Type : Original Article (s)

Authors
Zia Fallah Mohammadi 1
Razieh Mohammadi 2
Jalil Aslani 2
1 Associate Professor, Department of Exercise Physiology, School of Physical Education and Sports Sciences, University of Mazandaran, Babolsar, Iran
2 MSc Student, School of Physical Education and Sports Sciences, University of Mazandaran, Babolsar, Iran
Abstract
Background: The purpose of this study was to investigate the effects of extract of Eriobotrya japonica flower on brain-derived neurotrophic factor (BDNF), superoxide dismutase (SOD), and malondialdehyde (MDA) levels in the hippocampus of Parkinsonian rats after 12 weeks of voluntary exercise on a running wheel.Methods: 43 rats were divided into six groups of healthy control, Parkinsonian control, training group, Parkinsonian training, extract Parkinsonian, and training-extract Parkinsonian. Training group exercised on running wheels for 12 weeks. Training-extract group exercised on running wheels and received 200 mg/kg extract of Eriobotrya japonica intraperitoneally three times per week during study period. To induce Parkinson, 6-hydroxydopamine (6-OHDA) (dissolved in saline) was administered intracerebrovenricular (ICV) by a stereotaxic apparatus. BDNF level were measured using the enzyme-linked immunosorbent assay (ELISA) test. SOD and MDA levels were measured using spectrophotometric method. Data was analyzed using one-way analysis of variance (ANOVA) and least significant difference (LSD) post-hoc tests.Findings: Exercise in Parkinsonian-training and extract in Parkinsonian-extract group could not prevent decrease of BDNF and difference between healthy control and training-extract group was significant (P = 0.001). Voluntary wheel running and extract significantly prevented SOD level to decrease in Parkinsonian rats (P = 0.001). Differences of SOD level between training-extract and healthy group were not significant (P = 0.125). Training and extraction could not prevent MDA level to increase compared with healthy control group (P = 0.918). The difference of MDA level between extract-Parkinsonian and healthy group was not significant (P = 0.904).Conclusion: Pre-treatment with voluntary exercise and extraction of Eriobotrya japonica increase oxidative protection capacity of hippocampus against 6-OHDA toxicity but they cannot prevent significant decrease of BDNF level against 6-OHDA. Possibly, along with increase of SOD and decrease of MDA there would be no need to BDNF increase in order to counteract oxidative stress induced by exercise.
Keywords
Eriobotrya japonica
Voluntary exercise
6-hydroxydopamine
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Journal of Isfahan Medical School
Volume 32, Issue 274 - Serial Number 274
January and February 2014
Pages 120-130

  • Receive Date 27 July 2013
  • Accept Date 06 April 2022