Journal of Isfahan Medical School

Journal of Isfahan Medical School

Effect of Intraperitoneal Injection of Lipopolysaccharide (LPS) on Apoptosis, Memory and Alzheimer’s Disease Processes in the Hippocampus of Male Wistar Rats in a Time-Dependent Manner

Document Type : Original Article (s)

Authors
Shahrbanoo Oryan 1
Morteza Nazari-Serenjeh 2
Masoud Bagherpoor-Zarchi 2
Fariba Khodagholi 3
Mohammad Nabiuni 4
Kiana Shahzamani 5
1 Associate Professor, Department of Animal Physiology, School of Biology, Kharazmi University, Tehran, Iran
2 Department of Animal Physiology, Schoo of Biology, Kharazmi University, Tehran, Iran
3 Associate Professor, Department of Cellular and Molecular Biology, School of Biology, Kharazmi University, Tehran, Iran
4 Assistant Professor, Department of Cellular and Molecular Biology, School of Biology, Kharazmi University, Tehran, Iran
5 Assistant Professor, Department of Biology, School of Science, Lorestan University, Khorramabad, Iran
Abstract
Background: In this study, the effects of intraperitoneal injection of lipopolysaccharide (LPS) in causing apoptosis by increasing the amount of protein apoptotic B-cell lymphoma-2- associated X protein (Bax) and Caspase-3 and decreasing protein anti-apoptotic B-cell lymphoma-2 (bcl-2) in the hippocampus of rats and its effect on memory in a time-dependent manner at 12, 24, 48, 72 and 120 hours after injection were examined.Methods: Male Wistar rats of 230 to 250 g for Western blotting, enzymatic test and shuttle box were used.Findings: Increase in the protein content of apoptotic Bax and Caspase-3 and decrease in antiapoptotic bcl-2 protein in a time-dependent manner was observed in cases compared to the controls. The results of testing the enzyme Caspase-3 confirmed Western blotting results. Dysfunction caused by LPS in memory determined by shuttle box test. All the results were significant at the level of P ˂ 0.001.Conclusion: Results showed that LPS increased the incidence of apoptosis and neuronal cell death in the hippocampus region of the brain of male Wistar rats in a time-dependent manner and decrease animal's memory.
Keywords
Alzheimer's disease
Apoptosis
Shuttle box
Lipopolysaccharide
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Journal of Isfahan Medical School
Volume 32, Issue 285 - Serial Number 285
March and April 2014
Pages 650-659

  • Receive Date 09 March 2014
  • Accept Date 06 April 2022