Mechanical Behavior Obtaining of Cardiac Contracting Cell, Using Atomic Force Microscopy (AFM) and Analytical Relations

Document Type : Original Article (s)

Authors

1 Department of Biomechanics, School of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran

2 Associate Professor, Department of Biomechanics, School of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran

3 Associate Professor, Department of Hematology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Abstract

Background: Cardiac contracting cells cause heart pumping motion by its contraction and any problem in this motion is related to these cells. In one approach, considering cardiac cell as a homogenous body with linear elastic properties, it can be analytically mechanically modeled. To obtain cell elastic modulus by existing analytical relations, experimental data are needed.Methods: In this study, suitable experimental data were collected from mouse cardiac cells using atomic force microscopy (AFM). Then, by contact mechanics relations for this experimental method, elastic modulus were obtained and analyzed with two methods.Findings: Using two methods, elastic moduli were 48.08 ± 2.26 and 55.67 ± 2.56 kPa. Variations of elastic modulus by indentation depth showed nonlinear elastic properties of these cells.Conclusion: It can be concluded that via both of the presented methods, the same results for elastic modulus value for these contracting cells were obtained approximately. Also, it could be said that the linear Hertz’s model is suitable for initial prediction of the cell mechanical properties and to more exact and general evaluate of these properties, it is better to apply nonlinear models.

Keywords

References

  1. Tsamis A, Bothe W, Kvitting JP, Swanson JC, Miller DC, Kuhl E. Active contraction of cardiac muscle: in vivo characterization of mechanical activation sequences in the beating heart. J Mech Behav Biomed Mater 2011; 4(7): 1167-76.
  2. Baohua Ji, Gang Bao. Cell and molecular biomechanics: perspectives and challenges. ACTA MECH SOLIDA SIN 2011; 24(1): 27-51.
  3. Davidson L, von DM, Zhou J. Multi-scale mechanics from molecules to morphogenesis. Int J Biochem Cell Biol 2009; 41(11): 2147-62.
  4. Kuznetsova TG, Starodubtseva MN, Yegorenkov NI, Chizhik SA, Zhdanov RI. Atomic force microscopy probing of cell elasticity. Micron 2007; 38(8): 824-33.
  5. Franz CM, Puech PH. Atomic Force Microscopy: A versatile tool for studying cell morphology, adhesion and mechanics. Cell Mol Bioeng 2008; 1(4): 289-300.
  6. Webb HK, Truong VK, Hasan J, Crawford RJ, Ivanova EP. Physico-mechanical characterisation of cells using atomic force microscopy-current research and methodologies. J Microbiol Methods 2011; 86(2): 131-9.
  7. Zhou ZL, Ngan AH, Tang B, Wang AX. Reliable measurement of elastic modulus of cells by nanoindentation in an atomic force microscope. J Mech Behav Biomed Mater 2012; 8: 134-42.
  8. Li QS, Lee GY, Ong CN, Lim CT. AFM indentation study of breast cancer cells. Biochem Biophys Res Commun 2008; 374(4): 609-13.
  9. Mathur AB, Collinsworth AM, Reichert WM, Kraus WE, Truskey GA. Endothelial, cardiac muscle and skeletal muscle exhibit different viscous and elastic properties as determined by atomic force microscopy. J Biomech 2001; 34(12): 1545-53.
  10. Lieber SC, Aubry N, Pain J, Diaz G, Kim SJ, Vatner SF. Aging increases stiffness of cardiac myocytes measured by atomic force microscopy nanoindentation. Am J Physiol Heart Circ Physiol 2004; 287(2): H645-H651.
  11. Rico F, Roca-Cusachs P, Gavara N, Farre R, Rotger M, Navajas D. Probing mechanical properties of living cells by atomic force microscopy with blunted pyramidal cantilever tips. Phys Rev E Stat Nonlin Soft Matter Phys 2005; 72(2 Pt 1): 021914.
Journal of Isfahan Medical School
Volume 32, Issue 290 - Serial Number 290
May and June 2014
Pages 947-956

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History

  • Receive Date: 05 September 2013
  • Revise Date: 01 May 2024
  • Accept Date: 06 April 2022

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