Identifying Ubiquitin D in Paraffin-Embedded Tissues in Patients with Colorectal Cancer

Document Type : Original Article (s)

Authors

1 Young Researchers and Elite Club, Islamic Azad University, East Tehran Branch, Tehran, Iran

2 Assistant Professor, Department of Biology, School of Basic Sciences, Islamic Azad University, East Tehran Branch, Tehran, Iran

3 Department of Medical Genetics, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

4 Associate Professor, Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran

5 Assistant Professor, Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan AND Banej Exir Research Center, Tehran, Iran

6 Department of Hematology, Immunology Laboratory, Sajad Hospital AND Banej Exir Research Center, Tehran, Iran

Abstract

Background: Colon cancer is one major cause for cancer-related deaths. Ubiquitin D (UBD) and its alike proteins are signal transduction pathways with several cellular functions including DNA repair control. There is relation between increase in ubiquitin D and increase in p53 expression in a way that in many cases, this growth has accelerated the cancer progress. This study aimed to detect ubiquitin D, as a marker for the early detection of colorectal cancer and disease progress in patients.Methods: 30 samples of paraffin-embedded tissue blocks of patients with colorectal cancer and 10 normal tissue samples were studied. Reverse transcription-Polymerase chain reaction (RT-PCR) testing to detect ubiquitin D gene and b-actin gene as an internal control was obtained.Findings: The size of RT-PCR product of ubiquitin D gene was 395 and the size of amplified b-actin was 350 bp. The RT-PCR of b-actin was positive in all samples. The RT-PCR products of 18 samples (95%) of male patients and 11 samples (100%) of female patients were positive. Only one sample had negative product.Conclusion: Considering the results, we can conclude that ubiquitin D, as a useful marker that is independent of tumor stage, plays an important role in the detection of colorectal cancer and its study is really important in identification and detection of early stages of disease. Results showed that the expression of the ubiquitin D in patients is 97% and it reduces to 5% in healthy people.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 291 - Serial Number 291
May and June 2014
Pages 972-981

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History

  • Receive Date: 24 March 2014
  • Revise Date: 02 May 2024
  • Accept Date: 06 April 2022

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