Preventive Impacts of Selenium-Enriched Yeast on Liver and Enzyme Activity after Inducing Colorectal Cancer in Rat

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Biology, School of Science, Urmia University, Urmia, Iran

2 Assistant Professor, Department of Pathobiology and Quality Control, Artemia and Aquatic Research Institute, Urmia University, Urmia, Iran

3 Associate Professor, Department of Biology, School of Science, Urmia University, Urmia, Iran

4 Associate Professor, Department of Pathobiology, School of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract

Background: Colorectal cancer is the most common cause of death in the world. More studies were done on using the probiotics and selenium for cancer prevention. In this research, liver enzymes (such as alanine aminotransferase, asparate aminotransferase and alkaline phosphatase) changes were assessed following dietary administration of Saccharomyces cerevisiae (simple and selenium-enriched forms) in the rat with colorectal cancer induced by dimethyl hydrazine.Methods: Forty female rats, with 200-250 g initial body weight, were divided into five groups as healthy control, cancer control, simple form of yeast, selenium and selenium-enriched yeast. All animals received carcinogenic agent (40 mg/kg body weight) twice weekly for five weeks, except healthy controls which only received normal saline. Selenium group received 4 mg/ml of selenium nitrate in water; Saccharomyces cerevisiae was administered at the concentration of 5 × 108 CFU/ml with dimethyl hydrazine in yeast group; the last group received selenium-enriched yeast at the same concentration. At the end of forty weeks after Dimethyl hydrazine injection, all animals were euthanized and blood samples were taken for enzymes assay.Findings: Although the mean of enzymes activity were higher at cancer control, other groups significantly showed lower enzymes activity (P < 0.05). The most decrease of enzyme activity was seen in animals that received selenium-enriched yeast.Conclusion: The level of liver enzymes activity increases in colorectal cancer induced by dimethyl hydrazine in rat. Selenium-enriched yeast administration in rat could decreases the enzymes level and prevent tissue damage after carcinogenic agent injection.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 291 - Serial Number 291
May and June 2014
Pages 991-1003

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History

  • Receive Date: 25 November 2013
  • Revise Date: 02 May 2024
  • Accept Date: 06 April 2022

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