Journal of Isfahan Medical School

Journal of Isfahan Medical School

Screening LRTOMT Gene (DFNB63 locus) in Patients with Recessive Nonsyndromic Hearing Loss in Hormozgan Province, Iran

Document Type : Original Article (s)

Authors
Shahrbanoo Parchami-Barjui 1
Somayeh Reiisi 2
Fatemeh Rezaiean 3
Fatemeh Heybati 3
Morteza Hashemzadeh-Chaleshtori 4
1 Department of Biotechnology, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 PhD Student, Department of Genetics, National Institute for Genetic Engineering and Biotechnology, Tehran, Iran
3 MSc Student, Department of Genetics, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
4 Professor, Cellular and Molecular Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran
Abstract
Background: Congenital hearing loss is the most common sensory disorder is modern societies. Two modes of syndromic and nonsyndromic genetic hearing loss can be seen. Autosomal recessive non-syndromic inheritance hearing loss (ARNSHL) is the most common form of inheritance hearing loss. So, far mapping it, 95 loci of the 41 regions of deafness genes have been identified. Despite numerous studies in this field, for DFNB63 (Leucine rich transmembrane and O-methyltransferase or LRTOMT gene), few studies have been conducted. Thus, the locus mutations can help us to better understand the genes involved in hearing loss.Methods: 90 cases of hearing loss in Hormozgan province, Iran, were studied. DNA extracted via phenol-chloroform method. Polymerase chian reaction (PCR) was performed. Then, the product was used to perform polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) and polymerase chain reaction-heteroduplex analysis (PCR-HA) methods. Samples with different bands were sequenced to determine the nucleotide changes.Findings: In different exons, 8 samples (each in a specific exon) were sequenced to determine the type of changes that shift single-strand conformation polymorphism bands. No change was found in nucleotide sequencing of exons in any of the groups.Conclusion: The results showed no relationship between non-syndromic deafness and LRTOMT gene mutations. As this gene is discovered in recent years, there are few studies on it. So far, only 5 mutation of this gene have been identified in the world that can determine pale relationship of the gene and hearing loss.
Keywords
Mutation
PCR-HA
PCR-SSCP
Leucine rich transmembrane and O-methyltransferase (LRTOMT) gene
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Journal of Isfahan Medical School
Volume 32, Issue 298 - Serial Number 298
July and August 2014
Pages 1330-1337

  • Receive Date 10 March 2014
  • Accept Date 06 April 2022