Evaluation of Various Water Resources in Isfahan, Iran, for the Presence of Helicobacter Pylori Using Fluorescent Nested Polymerase Chain Reaction

Document Type : Original Article (s)

Authors

1 Assistant Professor, Department of Microbiology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Associate Professor, Department of Genetics and Molecular biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Helicobacter pylori infection is one of the most common infectious agents worldwide. However, origin and the mode of transmission of this bacterium have not been clearly explained. One of the most probable routs of Helicobacter pylori transmission is through water resources. The aim of this study was to evaluate the presence of Helicobacter pylori in tap waters, well waters and Zayandeh Rood River collected from various locations in Isfahan, Iran.Methods: Totally, 100 liters of water were collected from Zayandeh Rood River, wells and drinking water pipeline network in the north, west, south and west locations of Isfahan city. Collected waters first subjected to filteration through 0.25 µm, then, filters were washed with phosphate buffered saline (PBS) and the washed out PBS used for DNA extraction. For DNA extraction, QiAamp DNA Mini Kit was used, fluorescent nested polymerase chain reaction was used for detection of Helicobacter pylori genome using UreC, 16srRNA and adhesion gene specific primers.Findings: Our results showed Helicobacter pylori infection in Zayandeh Rood river, well waters and drinking water from eastern region of Isfahan.Conclusion: Using primers srRNA16, UreC, fluorescent nested polymerase chain reaction method and fluorochromes 5CY, very little number of bacteria were identified.

Keywords

References

  1. Mendall MA, Goggin PM, Molineaux N, Levy J, Toosy T, Strachan D, et al. Relation of Helicobacter pylori infection and coronary heart disease. Br Heart J 1994; 71(5): 437-9.
  2. Horiuchi T, Ohkusa T, Watanabe M, Kobayashi D, Miwa H, Eishi Y. Helicobacter pylori DNA in drinking water in Japan. Microbiol Immunol 2001; 45(7): 515-9.
  3. Parsonnet J, Shmuely H, Haggerty T. Fecal and oral shedding of Helicobacter pylori from healthy infected adults. JAMA 1999; 282(23): 2240-5.
  4. Vyse AJ, Gay NJ, Hesketh LM, Andrews NJ, Marshall B, Thomas HI, et al. The burden of Helicobacter pylori infection in England and Wales. Epidemiol Infect 2002; 128(3): 411-7.
  5. Brown LM. Helicobacter pylori: epidemiology and routes of transmission. Epidemiol Rev 2000; 22(2): 283-97.
  6. Fox JG. The non-H pylori helicobacters: their expanding role in gastrointestinal and systemic diseases. Gut 2002; 50(2): 273-83.
  7. Mazari-Hiriart M, Lopez-Vidal Y, Calva JJ. Helicobacter pylori in water systems for human use in Mexico City. Water Sci Technol 2001; 43(12): 93-8.
  8. Klein PD, Graham DY, Gaillour A, Opekun AR, Smith EO. Water source as risk factor for Helicobacter pylori infection in Peruvian children. Gastrointestinal Physiology Working Group. Lancet 1991; 337(8756): 1503-6.
  9. Hulten K, Han SW, Enroth H, Klein PD, Opekun AR, Gilman RH, et al. Helicobacter pylori in the drinking water in Peru. Gastroenterology 1996; 110(4): 1031-5.
  10. Hegarty JP, Dowd MT, Baker KH. Occurrence of Helicobacter pylori in surface water in the United States. J Appl Microbiol 1999; 87(5): 697-701.
  11. Hulten K, Enroth H, Nystrom T, Engstrand L. Presence of Helicobacter species DNA in Swedish water. J Appl Microbiol 1998; 85(2): 282-6.
  12. Baker KH, Hegarty JP. Presence of Helicobacter pylori in drinking water is associated with clinical infection. Scand J Infect Dis 2001; 33(10): 744-6.
  13. Enroth H, Engstrand L. Immunomagnetic separation and PCR for detection of Helicobacter pylori in water and stool specimens. J Clin Microbiol 1995; 33(8): 2162-5.
  14. Findlay I, Matthews PL, Mulcahy BK, Mitchelson K. Using MF-PCR to diagnose multiple defects from single cells: implications for PGD. Mol Cell Endocrinol 2001; 183(Suppl 1): S5-12.
  15. Cellini L, Del VA, Di CM, Di CE, Favaro M, Donelli G. Detection of free and plankton-associated Helicobacter pylori in seawater. J Appl Microbiol 2004; 97(2): 285-92.
  16. Fujimura S, Kato S, Kawamura T. Helicobacter pylori in Japanese river water and its prevalence in Japanese children. Lett Appl Microbiol 2004; 38(6): 517-21.
  17. Khan A, Farooqui A, Kazmi SU. Presence of Helicobacter pylori in drinking water of Karachi, Pakistan. J Infect Dev Ctries 2012; 6(3): 251-5.
  18. Al-Sulami AA, Al-Taee AM, Juma'a MG. Isolation and identification of Helicobacter pylori from drinking water in Basra governorate, Iraq. East Mediterr Health J 2010; 16(9): 920-5.
  19. Bahrami AR, Rahimi E, Ghasemian SH. Detection of Helicobacter pylori in city water, dental units' water, and bottled mineral water in Isfahan, Iran. ScientificWorldJournal 2013; 2013: 280510.
  20. Janzon A, Sjoling A, Lothigius A, Ahmed D, Qadri F, Svennerholm AM. Failure to detect Helicobacter pylori DNA in drinking and environmental water in Dhaka, Bangladesh, using highly sensitive real-time PCR assays. Appl Environ Microbiol 2009; 75(10): 3039-44.
  21. Giao MS, Azevedo NF, Wilks SA, Vieira MJ, Keevil CW. Persistence of Helicobacter pylori in heterotrophic drinking-water biofilms. Appl Environ Microbiol 2008; 74(19): 5898-904.
  22. Percival SL, Thomas JG. Transmission of Helicobacter pylori and the role of water and biofilms. J Water Health 2009; 7(3): 469-77.
Journal of Isfahan Medical School
Volume 32, Issue 298 - Serial Number 298
July and August 2014
Pages 1347-1353

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History

  • Receive Date: 04 August 2014
  • Revise Date: 05 May 2024
  • Accept Date: 06 April 2022

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