The Effect of Estradiol on Production of Type II Collagen and Aggrecan in Chondrogenic Proccess

Document Type : Original Article(s)

Authors

1 Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan , Iran

2 Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

3 Assistant Professor, Department of Anatomical Sciences, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran

Abstract

Background: Sex hormones play important role in proliferation, differentiation, maturation and the scheduled death of chondrocytes. Although, some studies report the regulatory role of estrogen in the development and progression of cartilage, some of the mechanisms still remain unclear, including the role of estrogen in the expression of cartilage-specific genes in chondrogenesis process. We studied this role in the present study.Methods: We used adipose-derived stem cells, which were previously differentiated into cartilage tissue in pellet culture system in the control (without estrogen in culture medium) and experimental (with estrogen in culture medium) groups. Production of chondrogenesis markers, type II collagen and aggrecan were evaluated in experimental and control groups via immunohistochemical (IHC) technique. Then, the results were evaluated with Image-J software and statistical analysis were done.Findings: Estrogen led to decrease of type II collagen and increase of aggrecan production.Conclusion: This study showed that chondrogenesis could be affected by estrogen.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 299 - Serial Number 299
July and August 2014
Pages 1371-1378

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History

  • Receive Date: 19 May 2013
  • Revise Date: 28 April 2024
  • Accept Date: 06 April 2022

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