In-vitro Therapeutic Effect of Allium Cepa, Oliveria Decumbens Vent and Muscari Neglectum against Trichomonas Vaginalis

Document Type : Original Article (s)

Authors

1 Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

2 Assistant Professor, Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

3 Professor, Department of Parasitology, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

4 Assistant Professor, Department of Nutrition, School of Medicine, Kashan University of Medical Sciences, Kashan, Iran

Abstract

Background: Trichomonas vaginalis is a flagellate pathogenic protozoon of human urogenital tract that infect 170 million new cases through the word annually. Considering the side effects of metronidazole, as common drug to treatment of this disease, this study was conducted to evaluate the in-vitro activity of extracts of Allium cepa, Oliveria decumbens Vent and Muscari neglectum against Trichomonas vaginalis.Methods: This experimental study was carried out on Trichomonas vaginalis isolated from patients referred to five health centers in Kashan, Iran, in 2013. The effect of alcoholic extract of Allium cepa, Oliveria decumbens Vent and Muscari neglectum on growth of Trichomonas vaginalis in concentrations of 12.5, 25, 50, 100, 200, 400, 800 and 1000 µg/ml was evaluated in TYI-s-33 medium after 12, 24 and 48 hours. The results were compared with metronidazole effect in concentrations of 0.025, 0.05, 0.1, 0.2, 0.4 µg/ml, as positive control. The number of alive and dead throphozoite measured and compared using trypan blue staininig. The half maximal inhibitory concentration (IC50) was determined using GraphPad prism5.Findings: The alcoholic extracts of Allium cepa, Oliveria decumbens Vent and Muscari neglectum had inhibitory effect on in-vitro growth of Trichomonas vaginalis. The IC50 rate was calculated 101.8 for Olivera dacumbens Vent, 572.3 for Allium cepa and 329.4 for Muscari neglectum after 24 hours. The IC50 rate of mrtonidazole was calculated 0.0326 at the same time.Conclusion: Alcoholic extracts of Allium cepa, Olivera dacumbens Vent and Muscari neglectum have antitrichomonal effects; the effect of Oliveria decumbens Vent was higher than others. We recommend further studies using major components of these plants, especially Olivera dacumbens, to investigate the antitrichomoniasis effect of it.

Keywords

References

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Journal of Isfahan Medical School
Volume 32, Issue 310 - Serial Number 310
September and October 2015
Pages 1958-1992

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History

  • Receive Date: 13 August 2014
  • Revise Date: 07 May 2024
  • Accept Date: 06 April 2022

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