Journal of Isfahan Medical School

Journal of Isfahan Medical School

Heterogeneity of Aminoglycoside Resistance Patterns among Fecal Escherichia Coli Strains Isolated from Animal and Human Sources in Alborz Province, Iran

Document Type : Original Article(s)

Authors
Hamed Moradi 1
Reza Ranjbar 2
Naser Harzandi 3
1 Department of Microbiology, Islamic Azad University, Karaj Branch, Karaj, Iran
2 Professor, Moulecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
3 Assistant Professor, Department of Microbiology, Islamic Azad University, Karaj Branch, Karaj, Iran
Abstract
Background: Escherichia coli is recognized as a major food-borne pathogen in humans worldwide. Antibiotic resistance of Escherichia coli isolates is a concern that has created widespread problem for treatment of infections caused by this bacterium. The aim of this study was to determine the pattern of aminoglycoside resistance among the strains of Escherichia coli isolated from animal and human sources in the Alborz province, Iran.Methods: The strains of Escherichia coli were recovered from keeping animals (dairies, beekeeping and sheep care center) and 2 laboratory facilities in Alborz province. These isolates were identified using standard biochemical and bacteriological tests. Susceptibility and antibiotic resistance of isolates were determined via disk diffusion method.Findings: Eighty isolates of Escherichia coli were isolated and included in the study. The total rates of antibiotic resistance of the isolates were as 72.5% to streptomycin, 13.75% to neomycin, and 6.25% to tobramycin and gentamicin; none of the isolates showed resistance to amikacin. Based on the source of the isolation, resistance rates to streptomycin was 95%, 85%, 65% and 45% in the isolates recovered from human, cow, chicken and sheep, respectively. The resistance rates to neomycin among the strains isolated from cow, sheep and chicken were 5%, 15% and 25%, respectively. Only 25% of the isolates recovered from chicken were resistant to gentamicin and tobramycin.Conclusion: This study showed a diversity of aminoglycoside resistance among isolates of Escherichia coli recovered from different sources. While aminoglycoside resistance of Escherichia coli is varied among different sources of isolation, continuous monitoring of resistance patterns and the use of antibiotic agents according to the sources of isolation is recommended.
Keywords
Escherichia coli
Aminoglycoside resistance
Antibiotic susceptibility testing
  1. Ryan KJ, Ray CG. Sherris medical microbiology. 4th ed. New York, NY: McGraw-Hill Medical; 2003. p. 343-73.
  2. American Academy of Microbiology. Antimicrobial resistance an ecological perspective. Washington, DC: American Society for Microbiology; 1999. p. 1-14.
  3. Brooks GF, Butel JS, Morse SA. Jawetz, Melnick, and Adelberg's medical microbiology. 23th ed. New York, NY: McGraw-Hill Medical; 2004. p. 256-61.
  4. Rao AN, Barlow M, Clark LA, Boring JR, III, Tenover FC, McGowan JE, Jr. Class 1 integrons in resistant Escherichia coli and Klebsiella spp., US hospitals. Emerg Infect Dis 2006; 12(6): 1011-4.
  5. Chang YC, Shih DY, Wang JY, Yang SS. Molecular characterization of class 1 integrons and antimicrobial resistance in Aeromonas strains from foodborne outbreak-suspect samples and environmental sources in Taiwan. Diagn Microbiol Infect Dis 2007; 59(2): 191-7.
  6. Vakulenko SB, Mobashery S. Versatility of aminoglycosides and prospects for their future. Clin Microbiol Rev 2003; 16(3): 430-50.
  7. Winn WC, Allen SD, Janda WM, Koneman EW, Procop GW, Schreckenberger PC. Koneman's color atlas and textbook of diagnostic microbiology. 6th ed. Philadelphia, PA: Lippincott Williams and Wilkins; 2005. p. 211-38.
  8. Menashe O, Kaganskaya E, Baasov T, Yaron S. Aminoglycosides affect intracellular Salmonella enterica serovars typhimurium and virchow. Antimicrob Agents Chemother 2008; 52(3): 920-6.
  9. Mohebi R, Taheri F, Saberi E. A study of antibiotic resistance pattern and plasmid profile of uropathogenic Escherichia coli isolated in Ilam (western Iran). J Ilam Univ Med Sci 2009; 17(2): 44-9. [In Persian].
  10. Milani M, Nahaei MR, Lotfipour F, Yousefee S. Antibiotic sensitivity of prevalent bacteria isolated from urinary tract infection during 1998-2005. Phrama Sci 2008; 4: 47-53.
  11. Eslami G, Eslami G, Eslami G, Fallah F, Goudarzi M. Distribution of integrons among multidrug resistant E. coli and klebsiella strains. Pajouhesh Dar Pezeshki 2010; 34(1): 61-5. [In Persian].
  12. Kang HY, Jeong YS, Oh JY, Tae SH, Choi CH, Moon DC, et al. Characterization of antimicrobial resistance and class 1 integrons found in Escherichia coli isolates from humans and animals in Korea. J Antimicrob Chemother 2005; 55(5): 639-44.
  13. Yang CM, Lin MF, Lin CH, Huang YT, Hsu CT, Liou ML. Characterization of antimicrobial resistance patterns and integrons in human fecal Escherichia coli in Taiwan. Jpn J Infect Dis 2009; 62(3): 177-81.
Journal of Isfahan Medical School
Volume 33, Issue 322 - Serial Number 322
January and February 2015
Pages 93-99

  • Receive Date 08 October 2014
  • Accept Date 06 April 2022