Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effects of Simultaneous Administration of Granulocyte Colony-Stimulating Factor and Interferon-alpha on Osteoporosis

Document Type : Original Article (s)

Authors
Roya Lari 1
Sima Jahan-Bakhshi 2
Elaheh Mohammadian 2
Ali Mirshahi 3
Nasser Mahdavi-Shahri 4
Ali Moghimi 4
1 Assistant Professor, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, Iran
2 MSc Student, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, Iran
3 Assistant Professor, Department of Clinical Sciences, School of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran
4 Professor, Department of Biology, School of Science, Ferdowsi University of Mashhad, Mashhad, Iran
Abstract
Background: Bone loss is one of the major pathological symptoms of rheumatoid arthritis (RA) and some types of cancers. Granulocyte colony-stimulating factor (G-CSF) and interferon-alpha (IFN-α) are widely used in patients with cancer as a drug and o have been known to play a key role in rheumatoid arthritis. This study aimed to investigate the effects of these factors on osteoporosis.Methods: 28 mouse (Mus musculus, male, and eight weeks of age) were divided into 4 groups of 7. They were injected subcutaneously with distilled water as control, 10 mg/kg IFN-α and 200 mg/kg G-CSF with or without 10 mg/kg IFN-α per day, for 28 days. After killing mice, standardized radiographs were taken from femur bones and the Image J program was used for measurement of the femoral bone density. The total femoral bone volume was measured using multidetector computed tomography (MDCT).Findings: G-CSF reduced the density of the head and greater trochanter of femural bone in mice. The IFN-a  alone increased bone density in the greater trochanter and the volume of the trabecular bone. This factor, in combination with G-CSF, strongly enhanced bone density and increased the volume of the trabecular bone.Conclusion: Therefore, administration of IFN-a may inhibit G-CSF-induced bone loss in inflammatory and treatment conditions.
Keywords
Osteoporosis
Interferon-α
Granulocyte colony-stimulating factor
Radiography
Multidetector computed tomography
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Journal of Isfahan Medical School
Volume 33, Issue 334 - Serial Number 334
March and April 2015
Pages 703-713

  • Receive Date 25 February 2015
  • Accept Date 06 April 2022