Comparing the Frequency of TIM-3 Polymorphism in Multiple Sclerosis Patients with Healthy Controls

Document Type : مقاله کوتاه

Authors

1 MSc Student, Department of Immunology, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

2 Assistant Professor, Cellular and Molecular Immunology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran

3 Associate Professor, Department of Cellular and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

4 Assistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

5 PhD Student, Department of Genetics, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

6 Professor, Department of Neurology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS) initiated and mediated by autoreactive T-helper1 cells directed against myelin antigens. One of the T-cell surface receptors is T-cell immunoglobulin and mucin domain (TIM) family. There are several single nucleotide polymorphisms (SNPs) in their sequences witch had associated with susceptibility to different autoimmune diseases. The aim of this study was to investigate the susceptibility of patients with multiple sclerosis in Isfahan population, Iran, with polymorphism +4259A>C in TIM-3 gene.Methods: Blood samples were collected. DNA was extracted from the blood samples using Genomic DNA Extraction Kit. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was performed. TIM-3 gene was amplified using PCR. Then, the products were digested with restriction enzyme, PstI. Electrophoresis was performed for separating the digested products.Findings: Genotype and allele carrier frequency between patient and healthy groups were statistically significant (P = 0.029). The odds ratio of susceptibility to multiple sclerosis for whom carrying C allele of TIM-3, compared with those who do not carry, was about 2 (P = 0.010).Conclusion: +4259A>C polymorphism in TIM-3 gene is associated with multiple sclerosis in Isfahan population, measuring the expression level of the genes carrying such polymorphisms and their relevant protein functional and/or structural analysis could be helpful.

Keywords

References

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Journal of Isfahan Medical School
Volume 33, Issue 341 - Serial Number 341
May and June 2015
Pages 1066-1075

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History

  • Receive Date: 26 April 2015
  • Revise Date: 06 May 2024
  • Accept Date: 06 April 2022

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