Journal of Isfahan Medical School

Journal of Isfahan Medical School

Detecting Metallo-Beta-Lactamase (MBL) Genes of blaSPM1, blaIMP1, blaIMP2, blaVIM 1, and blaVIM in Isolated Pseudomonas aeruginosa from Clinical Samples and its Antibiotic Resistance

Document Type : Original Article (s)

Authors
Mahdi Rouhi-Biroon 1
Kumarss Amini 2
Mahdi Parviz 3
1 Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
2 Assistant Professor, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
3 Instructor, Department of Microbiology, School of Basic Sciences, Saveh Branch, Islamic Azad University, Saveh, Iran
Abstract
Background: Pseudomonas aeruginosa is one of the important factors for nosocomial infections, particularly in patients with impaired immune systems and children. Carbapenems resistance, due to the presence of metallo-beta-lactamase (MBL) genes encoding enzymes, is considered as a serious threat. This study aimed to detect metallo-beta-lactamase genes encoding enzymes in Pseudomonas aeruginosa strains isolated from non-hospitalized patients and to assess its antibiotic resistance.Methods: Fifty strains of Pseudomonas aeruginosa were isolated and antibiogram test was investigated with 7 different antibiotics. For 8 imipenem-resistant strains, phenotypic testing was performed via double disk synergy test (DDST) and combine disk methods. Finally, to confirm phenotypic methods, multiplex polymerase chain reaction (PCR) test was performed for detection of target genes.Findings: According to the results of the antibiogram test, 16% of the samples were resistant to imipenem and 8% to meropenem or ceftizoxime. DDST and Combine disk methods did not found any beta-lactamase positive strain. Results of phenotypic methods were confirmed via multiplex PCR and no metallo-beta-lactamase specimen was identified.Conclusion: According to the study, meropenem and ceftizoxime are good alternative medicines for imipenem. As appropriate phenotypic and molecular methods showed, the metallo-beta-lactamase genes were not prevalent in non-hospitalized patients.
Keywords
Metallo-beta-lactamase
Multiplex polymerase chain reaction (PCR)
Pseudomonas aeruginosa
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Journal of Isfahan Medical School
Volume 33, Issue 343 - Serial Number 343
May and June 2015
Pages 1137-1146

  • Receive Date 29 April 2015
  • Accept Date 06 April 2022