The Effects of Boswellia Thurifera Aqueous Extract on the Structure of Testis Tissue and Sexual Hormonal Changes Following Administration of Endosulfan Toxin in Immature Male Rats

Document Type : Original Article (s)

Authors

1 Department of Biology, Kazerun Branch, Islamic Azad University, Kazerun, Iran

2 Associate Professor, Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

3 Associate Professor, Department of Physiology, Kazerun Branch, Islamic Azad University, Kazerun, Iran

Abstract

Background: Endosulfan toxin is an organochlorine pesticide reduces the ability of reproductive system. In traditional medicine, herbs are used for treatment of infertility. Boswellia thurifera herb is used to treat various diseases. This study aimed to evaluate the effects of Boswellia thurifera aqueous extract on the structure of testis and sexual hormonal changes following administration of endosulfan toxin.Methods: Forty immature male rats (aged 4-5 weeks) weighting 100-150 g, divided randomly to five equal groups. First group received only distilled water; the second group received 500 mg/kg/day Boswellia thurifera aqueous extract for 4 weeks; the third group received 10 mg/kg endosulfan pesticide for 2 weeks; and group IV and V received 10 mg/kg endosulfan pesticide for the first 2 weeks and then for 4 weeks 250 and 500 mg/kg/day aqueous extract of frankincense. The endosulfan pesticide and plant extract were administered once per each three days and daily via oral gavage, respectively. At the end of the experiment, the rats were anaesthetized with ether and blood sample was taken from the heart. Following luteinizing hormone (LH), follicle-stimulating hormone (FSH) and testosterone levels were measured. The testis were removed and stored in 10% formalin solution. The tissue samples were fixed and dehydrated with alcohol. Serial sections of 5 µm in thickness were cut using a microtome and stained using the Hematoxilin-Eosin method.Findings: The mean seminiferous tubular diameter, germinal layer, testosterone and FSH significantly increased in group IV compared to the group II (P < 0.05). Besides, the spermatogonias cells and Sertoli and Leydig cells significantly increased in groups III and IV compared to the group II (P < 0.05).Conclusion: The administration of aqueous extract of Boswellia thurifera probably decreased endosulfan effects; so that, significantly increased the activity of pituitary-testicular axis and improved the testis structure.

Keywords


  1. Mosher WD, Pratt WF. Fecundity and infertility in the United States: incidence and trends. Fertil Steril 1991; 56(2): 192-3.
  2. Matzuk MM, Lamb DJ. Genetic dissection of mammalian fertility pathways. Nat Cell Biol 2002; (4 Suppl): s41-s49.
  3. Sarvari A, Naderi MM, Heidari M, Zarnani AH, Jeddi-Tehrani M, Sadeghi MR, et al. Effect of environmental risk factors on human fertility. J Reprod Fertil 2010; 11(4): 211-25.
  4. Parandin R, Sadeghipour Rodsari HR, Shamili S, Ghasempour HR. Effects of aqueous extract of Boswellia thurifera on fertility in male rats. J Zanjan Univ Med Sci 2009; 16(65): 23-30. [In Persian].
  5. Tavakkolifar B, Massoudi M, Zarringhalam J. Review on pharmacological activities of gum olibanum. Journal of Medicinal Plants 2009; (32): 1-13. [In Persian].
  6. Behnamrasuli M, Hoseinzadh H, Ghafarimoghaniverdam G. The effects of Oliganum aqueous extraction during pregnancy and lactation on the learning behavior and memory near rat newborns. Journal of Science Kharazmi University 2001; 1(1): 1-13. [In Persian].
  7. Nusier MK, Bataineh HN, Bataineh ZM, Daradka HM. Effect of frankincense (Boswellia thurifera) on reproductive system in adult male rat. J Health Sci 2007; 53(4): 365-70.
  8. Mohammadi J, Chatrroz B, Delaviz H. The effect of hydroalcoholic extract of capparis spinosa on quality of sperm and rate of testosterone following induction of diabetes in rats. J Isfahan Med Sch 2014; 31(264): 2042-52. [In Persian].
  9. Krohn K, Rao MS, Raman NV, Khalilullah M. High-performance thin layer chromatographic analysis of anti-inflammatory triterpenoids from Boswellia serrata Roxb. Phytochem Anal 2001; 12(6): 374-6.
  10. Syrovets T, Buchele B, Gedig E, Slupsky JR, Simmet T. Acetyl-boswellic acids are novel catalytic inhibitors of human topoisomerases I and IIalpha. Mol Pharmacol 2000; 58(1): 71-81.
  11. Hillson RM. Gold, frankincense and myrrh. J R Soc Med 1988; 81(9): 542-3.
  12. Michie CA, Cooper E. Frankincense and myrrh as remedies in children. J R Soc Med 1991; 84(10): 602-5.
  13. Pandey RS, Singh BK, Tripathi YB. Extract of gum resins of Boswellia serrata L. inhibits lipopolysaccharide induced nitric oxide production in rat macrophages along with hypolipidemic property. Indian J Exp Biol 2005; 43(6): 509-16.
  14. Sharma RK, Agarwal A. Role of reactive oxygen species in male infertility. Urology 1996; 48(6): 835-50.
  15. Irvine DS. Epidemiology and aetiology of male infertility. Hum Reprod 1998; 13(Suppl 1): 33-44.
  16. Jamil K, Shaik AP, Mahboob M, Krishna D. Effect of organophosphorus and organochlorine pesticides (monochrotophos, chlorpyriphos, dimethoate, and endosulfan) on human lymphocytes in-vitro. Drug Chem Toxicol 2004; 27(2): 133-44.
  17. Soto AM, Sonnenschein C, Chung KL, Fernandez MF, Olea N, Serrano FO. The E-SCREEN assay as a tool to identify estrogens: an update on estrogenic environmental pollutants. Environ Health Perspect 1995; 103 (Suppl 7): 113-22.
  18. Modaresi M, Seif MR. Effects of endosulfan on the reproductive parameters of male rats. J Reprod Fertil 2011; 12(2): 117-22.
  19. Sinha N, Adhikari N, Saxena K. Effect of endosulfan during fetal gonadal differentiation on spermatogenesis in rats. Environ Toxicol Pharmacol 2001; 10(1-2): 29-32.
  20. Umar HA, Eze ED, Ahmed LI, Sherif AI, Malgwi S. Endosulfan-induced changes in sperm count, testicular weight and some erythrocyte indices in male guinea pigs. British Journal of Pharmacology and Toxicology 2012; 3(4): 151-5.
  21. Gaido K, Dohme L, Wang F, Chen I, Blankvoort B, Ramamoorthy K, et al. Comparative estrogenic activity of wine extracts and organochlorine pesticide residues in food. Environ Health Perspect 1998; 106(Suppl 6): 1347-51.
  22. Parandin R, Ghorbani R, Sadeghipour Roodsari HR. Effects of alcoholic extract of achillea millefolium flowers on fertility parameters in male rats. J Shaheed Sadoughi Univ Med Sci 2011; 19(1): 84-93. [In Persian].
  23. Modaresi M, Messripoor M, Asadi M, Morghmaleki MKH. The effect of Saffron extract on testis tissue. Iranian Journal of Medicinal and Aromatic Plants 2008; 24(2): 237-43. [In Persian].
  24. Naseri M, Heydari Nasrabadi M, Khodarahmi P, Ahmadi F, Mojibi P, Abotalebei H. Study of the effect of fumaria parviflora alcoholic extract on spermatogenesis in male rats. New Cellular and Mulecular Biotechnology Journal 2011; 1(2): 61-5. [In Persian].
  25. Ashraf H, Khaneshi F, Rafiee Raki F, Nejati V. Evaluation of aqueous extract of berberis integerrima root on the testis tissue and testosterone levels in stereptozotocine (STZ) induced diabetic rats. Qom Univ Med Sci J 2013; 7(4): 28-35. [In Persian].
  26. Mirfard M, Johari H, Mokhtari M, Hematkhah V, Jamali H, Allahverdi Gh. The effect of hydro-alcoholic garlic extract on testis weight and spermatogenesis in mature male rats under chemotherapy with cyclophosphamide. J Fasa Univ Med Sci 2011; 1(3): 123-30. [In Persian].
  27. Kazemi P, Jowhary H, Sharifi E, Zeraatpishe A. Androgenic effect of origanum vulgarel L.spp viride extract on hormone level of pituitary - gonadal axis in mature male vistar rats. J Arak Univ Med Sci 2012; 14(6): 89-96. [In Persian].
  28. Hemayatkhah Jahromi V, Parivar K, Forozanfar M. The effect of cinnamon extract on spermatogenesis hormonal axis of pituitary gonad in mice. Iranian Journal of Applied Animal Science 2011; 1(2): 99-103.
  29. Carlson BM. Human embryology and developmental biology. 3rd ed. Philadelphia, PA: Saunders; 2004.