Comparing the Expression of Foxa1 and Foxa2 Genes in the Stomach in Men and Women

Document Type : Original Article (s)

Authors

1 MSc Student, Department of Genetics, School of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

2 Assistant Professor, Department of Biology, School of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran

3 Assistant Professor, Department of Genetics, School of Medicine, Kazerun Branch, Islamic Azad University, Kazerun, Iran

Abstract

Background: In recent years, it is found that a large number of genes are differentially expressed in two sexes; this can be referred to sexual dimorphism in gene expression. One of the main reasons for the differences in gene expression between male and female is assigned to sex hormones. Regarding to the presence of estrogen and androgen response elements in the regulatory region of Foxa1 and Foxa2 genes and sexual dimorphism in the incidence of gastric cancer, in this study, we compared the expression of these genes in the stomach of healthy men and women.Methods: Sampling was done from 20 healthy men and 21 healthy women using endoscopy from the gastric antrum. Following RNA extraction and complementary DNA (cDNA) synthesis, expression of Foxa1 and Foxa2 genes was compared between the men and women using semi-quantitative technique of reverse transcription polymerase chain reaction (RT-PCR). Then, the data were analyzed using statistical t and ANOVA tests.Findings: The expression of Foxa1 was significantly higher in women than men (P = 0.041, df = 36, t = 2.12). In addition, the expression of this gene was significantly higher in people under the age of 45 years than people above it (P = 0.010, df = 36, t = 2.71). But about Foxa2 gene, no significantly results were seen.Conclusion: Foxa1 gene expression in women’s stomach is higher than men’s; besides, the expression of this gene in the stomach is decreased by age.

Keywords

References

  1. Saito K, Negishi M, James SE. Sexual dimorphisms in zonal gene expression in mouse liver. Biochem Biophys Res Commun 2013; 436(4): 730-5.
  2. Li Z, Tuteja G, Schug J, Kaestner KH. Foxa1 and Foxa2 are essential for sexual dimorphism in liver cancer. Cell 2012; 148(1-2): 72-83.
  3. Isensee J, Witt H, Pregla R, Hetzer R, Regitz-Zagrosek V, Noppinger PR. Sexually dimorphic gene expression in the heart of mice and men. J Mol Med (Berl) 2008; 86(1): 61-74.
  4. Gilbert JS, Nijland MJ. Sex differences in the developmental origins of hypertension and cardiorenal disease. Am J Physiol Regul Integr Comp Physiol 2008; 295(6): R1941-R1952.
  5. Sharma S, Eghbali M. Influence of sex differences on microRNA gene regulation in disease. Biol Sex Differ 2014; 5(1): 3.
  6. Campbell-Thompson M, Lynch IJ, Bhardwaj B. Expression of estrogen receptor (ER) subtypes and ERbeta isoforms in colon cancer. Cancer Res 2001; 61(2): 632-40.
  7. DeGraff DJ, Clark PE, Cates JM, Yamashita H, Robinson VL, Yu X, et al. Loss of the urothelial differentiation marker FOXA1 is associated with high grade, late stage bladder cancer and increased tumor proliferation. PLoS One 2012; 7(5): e36669.
  8. Danaei G, Vander HS, Lopez AD, Murray CJ, Ezzati M. Causes of cancer in the world: comparative risk assessment of nine behavioural and environmental risk factors. Lancet 2005; 366(9499): 1784-93.
  9. Jemal A, Murray T, Ward E, Samuels A, Tiwari RC, Ghafoor A, et al. Cancer statistics, 2005. CA Cancer J Clin 2005; 55(1): 10-30.
  10. Pricci M, Linsalata M, Russo F, Messa C, Amati L, Caradonna L, et al. Effects of 17beta-estradiol administration on apoptosis and polyamine content in AGS cell line. Anticancer Res 2001; 21(5): 3215-20.
  11. Camargo MC, Goto Y, Zabaleta J, Morgan DR, Correa P, Rabkin CS. Sex hormones, hormonal interventions, and gastric cancer risk: a meta-analysis. Cancer Epidemiol Biomarkers Prev 2012; 21(1): 20-38.
  12. Lin L, Miller CT, Contreras JI, Prescott MS, Dagenais SL, Wu R, et al. The hepatocyte nuclear factor 3 alpha gene, HNF3alpha (FOXA1), on chromosome band 14q13 is amplified and overexpressed in esophageal and lung adenocarcinomas. Cancer Res 2002; 62(18): 5273-9.
  13. Basseres DS, D'Alo F, Yeap BY, Lowenberg EC, Gonzalez DA, Yasuda H, et al. Frequent downregulation of the transcription factor Foxa2 in lung cancer through epigenetic silencing. Lung Cancer 2012; 77(1): 31-7.
  14. Ye DZ, Kaestner KH. Foxa1 and Foxa2 control the differentiation of goblet and enteroendocrine L- and D-cells in mice. Gastroenterology 2009; 137(6): 2052-62.
  15. Imamura Y, Sakamoto S, Endo T, Utsumi T, Fuse M, Suyama T, et al. FOXA1 promotes tumor progression in prostate cancer via the insulin-like growth factor binding protein 3 pathway. PLoS One 2012; 7(8): e42456.
  16. Naderi A, Meyer M, Dowhan DH. Cross-regulation between FOXA1 and ErbB2 signaling in estrogen receptor-negative breast cancer. Neoplasia 2012; 14(4): 283-96.
  17. Sahu B, Laakso M, Pihlajamaa P, Ovaska K, Sinielnikov I, Hautaniemi S, et al. FoxA1 specifies unique androgen and glucocorticoid receptor binding events in prostate cancer cells. Cancer Res 2013; 73(5): 1570-80.
  18. Tang Y, Shu G, Yuan X, Jing N, Song J. FOXA2 functions as a suppressor of tumor metastasis by inhibition of epithelial-to-mesenchymal transition in human lung cancers. Cell Res 2011; 21(2): 316-26.
  19. Chandanos E, Lagergren J. Oestrogen and the enigmatic male predominance of gastric cancer. Eur J Cancer 2008; 44(16): 2397-403.
  20. Lopes AM, Ross N, Close J, Dagnall A, Amorim A, Crow TJ. Inactivation status of PCDH11X: sexual dimorphisms in gene expression levels in brain. Hum Genet 2006; 119(3): 267-75.
  21. Murphy E, Steenbergen C. Gender-based differences in mechanisms of protection in myocardial ischemia-reperfusion injury. Cardiovasc Res 2007; 75(3): 478-86.
  22. Berchtold NC, Cribbs DH, Coleman PD, Rogers J, Head E, Kim R, et al. Gene expression changes in the course of normal brain aging are sexually dimorphic. Proc Natl Acad Sci U S A 2008; 105(40): 15605-10.
Journal of Isfahan Medical School
Volume 33, Issue 351 - Serial Number 351
July and August 2015
Pages 1564-1573

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History

  • Receive Date: 18 May 2015
  • Revise Date: 20 April 2024
  • Accept Date: 06 April 2022

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