Journal of Isfahan Medical School

Journal of Isfahan Medical School

Comparing the Effects of Transforming Growth Factor Beta1 (TGF-ß1) and Piascledine on the Expression of Collagen II, X and Aggrecan Genes in Chondrogenesis of human Adipose-Derived Stem Cells in Fibrin Alginate Composite Scaffold

Document Type : Original Article (s)

Authors
Hadi Didehvar 1
Farhad Golshan-Iranpoor 2
Ali Valiani 2
Batool Hashemibeni 3
Mojtaba Esmaeeli 1
1 MSc Student, Department of Anatomical Sciences, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Cartilage injuries are the leading cause of disability in the elderly in developed countries. In addition, articular cartilage has a limited ability to repair. Current treatment methods for cartilage tissue injuries lead to fibrous tissue formation. Cell therapy is a treatment in which stem cells using tissue engineering can be differentiated into chondrocytes by using growth factors and scaffolds. Since growth factors such as transforming growth factor beta1 (TGF-β) leads to hypertrophy of cartilage chondrocytes tissue and many scaffolds are weak in terms of mechanics and stability, it is essential to achieve the appropriate scaffolds and inducing factors. Studies have shown that fibrin alginate scaffold is appropriate in terms of mechanical and stability and piascledine increases the cartilage-specific genes expression. Therefore, in this study the chondrogenic effect of TGF-β1 and piascledine on adipose derived stem cells in fibrin alginate scaffold was compared and evaluated.Methods: Fat samples were obtained from three persons. Adipose derived stem cells (ADSCs) was extracted from adipose tissue and proliferated. Then the cells were transferred to the fibrin alginate scaffold and the cells were cultured for 21 days under the influence of the induction medium. The rate of proliferation and survival of cells was evaluated by [3 (4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium-bromide] MTT method and the rate of gene expression of Aggrecan and Collagen II and X was evaluated with Real-time polymerase chain reaction (Real-Time PCR) method.Findings: The results showed that proliferation rate and survival of cells in a fibrin alginate scaffold in the group containing Piascledine increased compared to the other groups, but this increase is not significant (P> 0.050). Also, Piascledine increased collagen II gene expression (P < 0.001) and reduced collagen X gene expression when compared to TGF-β1.Conclusion: Piascledine was found as a proper effective inducer in chondrogenic differentiation of human adipose derived stem cells cultured in fibrin alginate scaffold.
Keywords
Adipose derived stem cell
Piascledine
Chondrogenesis
Transforming growth factor beta1
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Journal of Isfahan Medical School
Volume 34, Issue 373 - Serial Number 373
January and February 2016
Pages 157-165

  • Receive Date 13 July 2015
  • Accept Date 06 April 2022