Journal of Isfahan Medical School

Journal of Isfahan Medical School

Prevalence of BCR-ABL Gene Fusions in Patients with Chronic Myeloid Leukemia with Multiplex Reverse Transcription Polymerase Chain Reaction

Document Type : Original Article (s)

Authors
Farzad Alizadeh-Mofrad 1
Mahdi Darikvand 1
Farid Dolatshahi 2
Parsa Mohammad-Jafari 1
Saber Agha-Mohammadi 1
1 Department of Biology, School of Basic Sciences, Islamic Azad University, Urmia Branch, Urmia ,Iran
2 Department of Biology, School of Basic Sciences, Islamic Azad University, Damghan Branch, Damghan,Iran
Abstract
Background: Chronic myeloid leukemia (CML) is a clonal malignancy of hematopoietic stem cells. Type of fusion in these patients can be clinically significant and help to better understand the pathogenesis of leukemic cells with t (9:22). The aim of this study is to check BCR-ABL mutations and determine the prevalence of various gene fusions using reverse transcription polymerase chain reaction (RT-PCR) method in patients with CML.Methods: After making sure of having the disease and signing the written consent, 5-8 ml blood was taken from patients with aseptic technique. After RNA extraction, blood samples were centrifuged to separate mononuclear cells (MNCs) and complete RNA was extracted. Multiplex reaction PCR was carried out in 35 cycles.Findings: All 40 patients were positive in terms of BCR-ABL fusions. Of them 21 persons (52.5%) had b3a2 fusion and 16 persons (38.88%) with b2a2 fusion and finally 3 persons with ale2 fusion.Conclusion: Uniformity of reaction products and the results obtained in this study showed that RT-PCR method is feasible as a fast, sensitive and safe method to determine the prevalence of BCR-ABL genes in the sampling of peripheral blood. Also, the difference in the rate of fusion due to racial differences in the population can be studied.
Keywords
Chronic myeloid Leukemia (CML)
Reverse transcription polymerase chain reaction (RT-PCR)
BCR-ABL ‎fusion
Iran
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Journal of Isfahan Medical School
Volume 34, Issue 382 - Serial Number 382
March and April 2016
Pages 515-520

  • Receive Date 12 November 2015
  • Accept Date 06 April 2022