Epstein-Barr Virus in Breast Carcinoma: A Study on an Iranian Population

Document Type : Original Article (s)

Authors

1 Professor, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran

2 Student of Medicine, School of Medicine AND Student Research Committee, Isfahan University of Medical Sciences, Isfahan, Iran

Abstract

Background: Invasive ductal carcinoma (IDC) is the most frequent type of breast carcinoma. Epstein-Barr virus is the most suspicious virus for the viral etiology of breast cancer. The aim of this study was to determine the expression of EBV nuclear antigen-1 (EBNA-1) in IDC.Methods: 40 Iranian women with invasive ductal carcinoma of breast were enrolled in this study. Tumors were assessed for the expression of EBNA1 antigen by PCR technique. Demographics of patients as well as characteristics of tumors including size, grade, and axillary lymph node status were collected and compared between EBNA1 positive and EBNA1 negative tumors.Findings: The mean age of patients was 48.7 year. EBNA-1 PCR assay was positive in 11 patients (27.5%). The mean diameter of tumors was 4.43 cm. Axillary lymph nodes were involved in 22 patients. Tumors were grade I, II, and III in 5, 19, and 16 patients, respectively. EBNA-1 positive tumors were significantly larger than EBNA-1 negative tumors (5.5 ± 2.6 vs. 4.0 ± 2.4; P = 0.049).Conclusion: According to the results of this study, EBV might have a role in the pathogenesis of breast cancer.

Keywords

References

  1. Mousavi SM, Montazeri A, Mohagheghi MA, Jarrahi AM, Harirchi I, Najafi M, et al. Breast cancer in Iran: an epidemiological review. Breast J 2007; 13(4): 383-91.
  2. El Saghir NS, Khalil MK, Eid T, El Kinge AR, Charafeddine M, Geara F, et al. Trends in epidemiology and management of breast cancer in developing Arab countries: a literature and registry analysis. Int J Surg 2007; 5(4): 225-33.
  3. Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D. Global cancer statistics. CA Cancer J Clin 2011; 61(2): 69-90.
  4. Martin AM, Weber BL. Genetic and hormonal risk factors in breast cancer. J Natl Cancer Inst 2000; 92(14): 1126-35.
  5. Hulka BS, Moorman PG. Breast cancer: hormones and other risk factors. Maturitas 2001; 38(1): 103-13.
  6. Serraino D, Piselli P, Scognamiglio P. Viral infections and cancer: epidemiological aspects. J Biol Regul Homeost Agents 2001; 15(3): 224-8.
  7. Antonsson A, Bialasiewicz S, Rockett RJ, Jacob K, Bennett IC, Sloots TP. Exploring the prevalence of ten polyomaviruses and two herpes viruses in breast cancer. PLoS One 2012; 7(8): e39842.
  8. Glenn WK, Heng B, Delprado W, Iacopetta B, Whitaker NJ, Lawson JS. Epstein-Barr virus, human papillomavirus and mouse mammary tumour virus as multiple viruses in breast cancer. PLoS One 2012; 7(11): e48788.
  9. Fina F, Romain S, Ouafik L, Palmari J, Ben AF, Benharkat S, et al. Frequency and genome load of Epstein-Barr virus in 509 breast cancers from different geographical areas. Br J Cancer 2001; 84(6): 783-90.
  10. Fukayama M, Hino R, Uozaki H. Epstein-Barr virus and gastric carcinoma: virus-host interactions leading to carcinoma. Cancer Sci 2008; 99(9): 1726-33.
  11. Hippocrate A, Oussaief L, Joab I. Possible role of EBV in breast cancer and other unusually EBV-associated cancers. Cancer Lett 2011; 305(2): 144-9.
  12. Le D, V, Tubiana-Hulin M, Friedman S, Hacene K, Spyratos F, Brunet M. Prognostic value of histologic grade nuclear components of Scarff-Bloom-Richardson (SBR). An improved score modification based on a multivariate analysis of 1262 invasive ductal breast carcinomas. Cancer 1989; 64(9): 1914-21.
  13. Xue SA, Lampert IA, Haldane JS, Bridger JE, Griffin BE. Epstein-Barr virus gene expression in human breast cancer: protagonist or passenger? Br J Cancer 2003; 89(1): 113-9.
  14. Mohamed WS, Mohamed MA, Omar MM. Possible involvement of Epstein-barr virus (EBV) in pathogenesis and prognosis of female breast infiltrating duct carcinoma: clinicopathological, immunohistochemical and molecular study. Egy J Med Microbiol 2007; 16(2): 403-14.
  15. Fawzy S, Sallam M, Awad NM. Detection of Epstein-Barr virus in breast carcinoma in Egyptian women. Clin Biochem 2008; 41(7-8): 486-92.
  16. Brink AA, van Den Brule AJ, van Diest P, Meijer CJ. Re: detection of Epstein-Barr virus in invasive breast cancers. J Natl Cancer Inst 2000; 92(8): 655-6.
  17. Grinstein S, Preciado MV, Gattuso P, Chabay PA, Warren WH, de Matteo E, et al. Demonstration of Epstein-Barr virus in carcinomas of various sites. Cancer Res 2002; 62(17): 4876-8.
  18. Herrmann K, Niedobitek G. Lack of evidence for an association of Epstein-Barr virus infection with breast carcinoma. Breast Cancer Res 2003; 5(1): R13-R17.
  19. Lorenzetti MA, de Matteo E, Gass H, Martinez VP, Lara J, Gonzalez P, et al. Characterization of Epstein Barr virus latency pattern in Argentine breast carcinoma. PLoS One 2010; 5(10): e13603.
  20. Glaser SL, Ambinder RF, DiGiuseppe JA, Horn-Ross PL, Hsu JL. Absence of Epstein-Barr virus EBER-1 transcripts in an epidemiologically diverse group of breast cancers. Int J Cancer 1998; 75(4): 555-8.
  21. Dadmanesh F, Peterse JL, Sapino A, Fonelli A, Eusebi V. Lymphoepithelioma-like carcinoma of the breast: lack of evidence of Epstein-Barr virus infection. Histopathology 2001; 38(1): 54-61.
  22. Kijima Y, Hokita S, Takao S, Baba M, Natsugoe S, Yoshinaka H, et al. Epstein-Barr virus involvement is mainly restricted to lymphoepithelial type of gastric carcinoma among various epithelial neoplasms. J Med Virol 2001; 64(4): 513-8.
  23. Zekri AR, Bahnassy AA, Mohamed WS, El-Kassem FA, El-Khalidi SJ, Hafez MM, et al. Epstein-Barr virus and breast cancer: epidemiological and molecular study on Egyptian and Iraqi women. J Egypt Natl Canc Inst 2012; 24(3): 123-31.
  24. Bonnet M, Guinebretiere JM, Kremmer E, Grunewald V, Benhamou E, Contesso G, et al. Detection of Epstein-Barr virus in invasive breast cancers. J Natl Cancer Inst 1999; 91(16): 1376-81.
  25. Murray PG, Lissauer D, Junying J, Davies G, Moore S, Bell A, et al. Reactivity with A monoclonal antibody to Epstein-Barr virus (EBV) nuclear antigen 1 defines a subset of aggressive breast cancers in the absence of the EBV genome. Cancer Res 2003; 63(9): 2338-43.
  26. Mazouni C, Fina F, Romain S, Ouafik L, Bonnier P, Brandone JM, et al. Epstein-Barr virus as a marker of biological aggressiveness in breast cancer. Br J Cancer 2011; 104(2): 332-7.
Journal of Isfahan Medical School
Volume 34, Issue 388 - Serial Number 388
May and June 2016
Pages 724-729

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History

  • Receive Date: 30 January 2016
  • Revise Date: 06 May 2024
  • Accept Date: 06 April 2022

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