Journal of Isfahan Medical School

Journal of Isfahan Medical School

Evaluation of the Frequency of Gene Polymorphism of Interleukin-7 Receptor Subunit Alpha (IL7R-α) in Atopic Patients and Healthy Controls

Document Type : Original Article (s)

Authors
Samaneh Mostafavi 1
Nahid Eskandari 2
Fereshteh Ale-Sahebfosoul 2
Mansour Salehi 3
Ramin Ghasemi 4
1 MSc Student, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Assistant Professor, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Associate Professor, Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Science, Isfahan, Iran
4 Subspecialist in Asthma, Allergy and Clinical Immunology, Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Atopy and/or allergy are in the same meaning. Patients with allergy are individuals who have positive family history and the number of eosinophil in their blood test and immunoglobulin E (IgE) level in their skin test are high against allergen, which is demonstrated by allergic rhinitis, eczema, asthma and urticaria. Many genetic factors are involved in disease onset one of which is interleukin-7 receptor subunit alpha (IL7R-α). In this study we aimed to evaluate the frequency of this polymorphism in two groups of case and control.Methods: 101 patients with allergy and 201 controls were selected. After DNA extraction, different genotypes of C/T polymorphisms were studied using high-resolution melt real-time polymerase chain reaction (HRM real-time PCR) technique.Findings: There was a significant association between the frequency of this gene polymorphism and atopy. In fact, individuals with TT genotype were approximately 4 times more at a risk of this disease.Conclusion: The frequency of this polymorphism with same results was also studied in both Germany and India, which shows that this polymorphism is significantly associated with allergy. Studies have also shown that IL-7 plays a pivotal role not only in eosinophil survival and operation but also in immune system homeostasis.
Keywords
Polymorphism
Interleukin-7 receptor subunit alpha (IL7R-α)
Atopy
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Journal of Isfahan Medical School
Volume 34, Issue 397 - Serial Number 397
July and August 2016
Pages 1019-1024

  • Receive Date 01 June 2016
  • Accept Date 06 April 2022