Journal of Isfahan Medical School

Journal of Isfahan Medical School

Prevalence Study of Aminoglycoside-Resistance Genes in Acinetobacter Baumannii Isolated from the Patients in Intensive Care Unit

Document Type : Original Article (s)

Authors
Fatemeh Amini 1
Hassan Ali Karimpour 2
Sivash Vaziri 3
Mahdi Ghaderi 4
Saeed Mohammadi 2
Mohsen Azizi 1
1 Research Assistant, Department of Microbiology, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Assistant Professor, Department of Anesthesiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Associate Professor, Department of Infectious Diseases, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
4 Department of Microbiology, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract
Background: Acinetobacter baumanniii is a highly antibiotic-resistant nosocomial opportunistic pathogen possess. This study aimed to assess the antibiotic-resistance pattern and detect the molecular frequency of aphA6 and aadB genes in Acinetobacter baumannii isolated from patients in intensive care unit (ICU).Methods: In this cross-sectional study, 58 Acinetobacter baumannii isolates collected from ICU patients in Imam Reza hospital, Kermanshah, Iran, were confirmed using standard bacteriological tests. Disc diffusion method was deployed for screening resistant isolates. The prevalence of aphA6 and aadB genes was assessed using polymerase chain reaction (PCR) method with specific primers.Findings: The highest antibiotic resistance rates were seen in ticarcilin (87.9%) and ceftazidime (86.2%) and the lowest was of polymyxin B (12.1%). Of 58 Acinetobacter isolates, 36 (62.1%) and 9 (15.5%) harbored aphA6 and aadB genes, respectively. In addition, 5 isolates (8.6%) harbored both genes and 18 isolates (31.3%) were negative for any of those genes.Conclusion: 80% of Acinetobacter isolates showed resistance to aminoglycoside antibiotics. Considering the high rates of antibiotic resistance to this category and other investigated antibiotics in this study, it seems necessary to precise on the use of antibiotics such as polymyxin B to treat the infections caused by this pathogen.
Keywords
Acinetobacter baumannii
Antibiotic Resistance
Aminoglycosides
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Journal of Isfahan Medical School
Volume 34, Issue 407 - Serial Number 407
September and October 2017
Pages 1381-1388

  • Receive Date 13 September 2016
  • Accept Date 06 April 2022