Journal of Isfahan Medical School

Journal of Isfahan Medical School

Detection of Macrolid, Lincosamide and Streptogramin Resistance Genes in Methicillin-Resistant Staphylococcus Epidermidis Strains Isolated from Clinical Samples in Isfahan City, Iran

Document Type : Original Article (s)

Authors
Mahtabossadat Mousavi 1
Vajihe Karbasizadeh 2
1 MSc Student, Department of Microbiology, School of Sciences, Falavarjan Branch, Islamic Azad University, Isfahan, Iran
2 Assistant Professor, Department of Bacteriology and Virology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
Abstract
Background: Increased antibiotic resistance among nosocomial infections has made the treatment of these infections difficult. Staphylococcus epidermidis is also known as one of the most common causes of nosocomial infections. The aim of this study was to determine the prevalence of resistance to macrolide, lincosamid and streptogramin antibiotics, in methicillin-resistant Staphylococcus epidermidis (MRSE) strains isolated from clinical samples and to detect their related resistance genes.Methods: For a period of 8 months, from 250 clinical Staphylococcus strains, 100 isolates of Staphylococcus epidermidis were identified and the antibiotic susceptibility patterns of the isolates were determined using the disc diffusion method. MRSE samples were isolated by using the disk diffusion method for cefoxitin. The minimum inhibitory concentration (MIC) of erythromycin was determined using the micro dilution method. The frequency of inducible clindamycin resistance phenotype was detected via D test method and the resistance genes to MLSB were detected using polymerase chain reaction (PCR) method.Findings: Among 100 Staphylococcus epidermidis strains, 52 isolates were MRSE. The frequency of resistance phenotype iMLSB, MS and cMLSB were 17.3%, 13.4% and 48%, respectively. The frequency of the resistance genes ermC, msrA and ermA were 73%, 11.5% and 5.7%, respectively.Conclusion: The high frequency of the ermC gene among isolates is a serious warning to health systems; thus, use of convenient and effective treatment methods after antibiotic susceptibility tests is necessary.
Keywords
Methicillin-resistant Staphylococcus epidermidis
erm genes
D test
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Journal of Isfahan Medical School
Volume 34, Issue 411 - Serial Number 411
November and December 2017
Pages 1507-1514

  • Receive Date 30 July 2016
  • Accept Date 06 April 2022