Journal of Isfahan Medical School

Journal of Isfahan Medical School

Prevalence Study of exoenzyme U (exoU) and exoenzyme S (exoS) Genes in Pseudomonas Aeruginosa Isolated from Burn Patients in Kermanshah City, Iran

Document Type : Original Article (s)

Authors
Kamal Ahmadi 1
Siavash Vaziri 2
Seyyed Hamidreza Mortazavi 3
Faizullah Mansouri 2
Mandana Afsharian 2
Ahmad Tajehmiri 4
Mahsa Kashef 1
Mohsen Azizi 1
1 Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
2 Associate Professor, Department of Infectious Diseases, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3 Assistant Professor, Department of Pediatrics, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
4 Medical Biology Research Center, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Abstract
Background: Pseudomonas aeruginosa is one of the major pathogens causing burnt wound infection in hospitals. This study aimed to evaluate the prevalence of exoenzyme U (exoU) and exoenzyme S (exoS) genes and the antibiotic resistance pattern of Pseudomonas aeruginosa isolated from burn patients' samples in Kermanshah City, Iran.Methods: In this cross-sectional study, 194 samples were tested with conventional bacteriological methods. After evaluation of antibiotic sensitivity with disc diffusion method, specific primers were deployed to assess the frequency of exoU and exoS genes among isolates. The retrieved data were analyzed using SPSS software.Findings: From 91 isolates of Pseudomonas aeruginosa, 72 (79.1%) were multi-drug resistant (MDR) isolates. The most prevalent antibiotic resistances were against gentamicin (79.1%) and ceftazidime (74.7%); the most prevalent sensitivities were against colistin (100%) and polymixin B (92.3%). The frequency of exoU and exoS genes was 80.2% and 68.1%, respectively. There were significant relationships between the frequency of exoU gene and resistance to ceftazidime (P = 0.041) and cefotaxime (P = 0.050).Conclusion: Considering the role of Pseudomonas aeruginosa in burn wound infections and the role of cytotoxin and antibiotic resistance genes in colonization and survival of this bacteria, avoiding home remedies, accurate detection of virulence factors, and recognition of antibiotic resistance pattern among the isolates in order to choose the appropriate antibiotic regimen to prevent infection seem to be necessary.
Keywords
Pseudomonas aeruginosa
اگزوآنزیم‌های U و S
مقاومت آنتی‌بیوتیکی
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Journal of Isfahan Medical School
Volume 35, Issue 428 - Serial Number 428
March and April 2017
Pages 496-502

  • Receive Date 14 February 2017
  • Accept Date 06 April 2022