Journal of Isfahan Medical School

Journal of Isfahan Medical School

Comparison of DNA Instability in Adenoma and Adenocarcinoma in Patients with Colorectal Cancer, Using Random Amplified Polymorphic DNA Polymerase Chain Reaction (RAPD-PCR)

Document Type : Original Article (s)

Authors
Ardeshir Talebi 1
Samsam Daneshbakhtiyar 2
Mahboobeh Meshkat 3
Marziyeh Meshkat 3
1 Associate Professor, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Resident, Department of Pathology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Department of Cellular and Molecular Biology, School of Sciences, Nourdanesh Institute of Higher Education, Meymeh, Isfahan, Iran
Abstract
Background: Colorectal cancer is the consequence of gathering numerous genetic alterations and it has been suggested that genomic instability is indispensable for the generation of multiple mutations underlying the development of cancer.Methods: Random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) method was utilized to find genomic alterations in adenomas and adenocarcinomas compared with normal epithelial tissue obtained from 17 patients with colorectal cancer.Findings: Separated PCR products by 2% agarose determined approximate 370 base pairs band as a polymorphic fingerprint for normal (11.8%), adenoma (76.5%), and adenocarcinoma (88.2%) tissues. Polymorphic band could significantly discriminate adenomas [Odds ratio (OR) = 24.38, P = 0.0004] and adenocarcinomas (OR = 56.25, P < 0.0001) from normal tissues. Furthermore, the 370 base pairs band could not distinguish adenomas from adenocarcinomas (P = 0.6600).
Keywords
Colorectal Cancer
Adenoma
Adenocarcinoma
Random amplified polymorphic DNA polymerase chain reaction
Genomic instability
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Journal of Isfahan Medical School
Volume 35, Issue 432 - Serial Number 432
March and April 2017
Pages 609-614

  • Receive Date 18 September 2016
  • Accept Date 06 April 2022