Journal of Isfahan Medical School

Journal of Isfahan Medical School

The Effect of Erythropoietin on Neuronal Apoptosis in Hippocampal Dentate Gyrus in Streptozotocin-Induced Alzheimer's Disease in Rats

Document Type : Original Article (s)

Authors
Moloud Shabrang 1
Bahman Rashidi 2
Hojjatallah Alaei 3
Mohammad Reza Sharifi 3
Parham Reisi 4
1 Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
2 Associate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
3 Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
4 Associate Professor, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
10.22122/jims.v35i454.8745
Abstract
Background: Alzheimer's disease is a disorder associated with neuronal degeneration in the areas involve in learning and memory. It has been proposed that erythropoietin probably has neuroprotective effects. Therefore, the aim of the present study was to evaluate the effect of erythropoietin on neuronal apoptosis in the granular layer of dentate gyrus of the hippocampus in an animal model of Alzheimer's disease.Methods: Animal model of Alzheimer's was established via bilateral intracerebroventricular (ICV) injection of streptozotocin (STZ). After 2 weeks and observation of cognitive disorder, rats received intraperitoneal (IP) injection of erythropoietin every other day with a dose of 5000 IU/kg for 2 weeks. Finally, the rats were anesthetized and their brains were dissected for immunohistochemical study. Terminal deoxynucleotidyl transferase biotin-dUTP Nick-End Labeling (TUNEL) method was used to study neuronal apoptosis.Findings: Intracerebroventricular injection of streptozotocin increased the number of apoptotic cells in the granular layer of dentate gyrus significantly (P < 0.05). Although erythropoietin had no effect on the control rats, it significantly reduced the number of apoptotic cells in the lesion group (P < 0.01)Conclusion: The results of this study indicate that erythropoietin may be effective in reducing the complications of neurodegenerative diseases by reducing neuronal apoptosis.
Keywords
Alzheimer's disease
Erythropoietin
Streptozotocin
Apoptosis
Hippocampus
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Journal of Isfahan Medical School
Volume 35, Issue 454 - Serial Number 454
September and October 2018
Pages 1593-1598

  • Receive Date 02 September 2017
  • Accept Date 06 April 2022