Journal of Isfahan Medical School

Journal of Isfahan Medical School

Evaluation of Interleukin-10 Gene Promoter Polymorphism (-819 C/T) in Patients with Chronic Hepatitis B Virus Infection

Document Type : Original Article (s)

Authors
Farzaneh Sadat Mirfakhar 1
Seyed Reza Mohebbi 2
Seyed Masoud Hosseini 3
Pedram Azimzadeh 4
Mahsa Saeedi-Niasar 5
Afsaneh Sharifian 6
Hamid Asadzadeh-Aghdaei 7
Mohammad Reza Zali 8
1 Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Assistant Professor, Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3 Professor, Department of Microbiology, School of Biological Sciences, Shahid Beheshti University, Tehran, Iran
4 PhD, Foodborne and Waterborne Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
5 Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
6 Associate Professor, Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
7 Assistant Professor, Basic and Molecular Epidemiology of Gastrointestinal Disorders Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
8 Professor, Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
10.22122/jims.v35i461.8934
Abstract
Background: Hepatitis B infection is a major epidemiological problem. The clinical outcome of hepatitis B virus infection varies between individuals from spontaneous viral clearance to chronic hepatitis. This diversity may be due to variations in genes encoding cytokines. Interleukin 10, as an anti-inflammatory cytokine, may have an effect on susceptibility to hepatitis B infection. The aim of this study was to investigate the role of interleukin-10 gene promoter polymorphism (-819 C/T) (rs1800871) as a possible factor for predicting susceptibility to chronic hepatitis B virus infection.Methods: Blood samples were collected from 122 patients with hepatitis B according to positive result of the enzyme-linked immunosorbent assay (ELISA) and 138 healthy subjects as control group. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay was used for polymorphism genotyping.Findings: There was a higher frequency of CT genotype among the patients with hepatitis B (50.8%) in comparison with healthy controls (32.6%). Statistically significant differences were found in the genotype (P = 0.007) and allele (P = 0.018) frequencies of interleukin-10 gene promoter polymorphism (-819 C/T) between the patients with hepatitis B and healthy controls.Conclusion: Our findings indicate that interleukin-10 gene promoter polymorphism (-819 C/T) may be associated with chronic hepatitis B virus infection and it can serve as a possible predictive factor for the development of chronic hepatitis B infection.
Keywords
Cytokine
Interleukin-10
Single Nucleotide Polymorphism
Chronic hepatitis B
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Journal of Isfahan Medical School
Volume 35, Issue 461 - Serial Number 461
November and December 2018
Pages 1852-1858

  • Receive Date 10 October 2017
  • Accept Date 06 April 2022