Evaluation of the Effects of Solamargine Extracted from Solanum Nigrum as an Angiogenesis Inhibitor

Document Type : Original Article (s)


1 Department of Biochemistry, School of Biology Science, Higher Education Institute of Rab-Rashid, Tabriz, Iran

2 Associate Professor, Biotechnology Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran


Background: Solamargine is one of asteroid-glycoalkaloids existing in Solanum nigrum Linn. Different studies have shown the cytotoxic effects of solamargine on the wide various cancer cell types. Angiogenesis is the factor that helps invasion and metastasis of tumors. Therefore, inhibition of angiogenesis is the target of many clinical treatments. In this study, we evaluated the effects of solamargine on inhibition of angiogenesis.Methods: The effects of solamargine on the angiogenesis was assessed using chicken chorioallantoic membrane (CAM) model that is a known method for studying angiogenesis in vivo. So, Ross fertilized eggs were divided into five groups including control, sham exposed [treated by phosphate buffered saline (PBS)], and treated with various concentrations of solamargine (5, 10, and 20 µM). At the end of each experiment, chicken chorioallantoic membranes were photographed by research photostereomicroscope, and the number and length of vessels were measured.Findings: Solmargine could significantly decrease the number and length of vessels in a dose-dependent manner. As, the most inhibitory was seen by solmargine 20 µM (number: 5.31 ± 1.80, length: 60.88 ± 7.31 µm) in compare with control group (number: 23.88 ± 2.67, length: 119.39 ± 8.87 µm).Conclusion: The results proposed that solmrgine has significant inhibitory effects on the angiogenesis.


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