Journal of Isfahan Medical School

Journal of Isfahan Medical School

Identification of Gene Mutation Patterns Obtained from Resistance to Mupirocin in Methicillin-Resistant Staphylococcus Aureus Clinical Strains, Using High-Resolution Melting (HRM) Method

Document Type : Original Article (s)

Authors
Hamed Tahmasebi 1
Sanaz Dehbashi 2
Mohammad Reza Arabestani 3
1 Department of Microbiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran
2 Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
3 Associate Professor, Brucellosis Research Center AND Department of Microbiology, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
10.22122/jims.v36i476.9995
Abstract
Background: Effective antibiotics on the translation pathway, such as mupirocin, may cause gene mutations in methicillin-resistant Staphylococcus aureus in long-term. Using high-sensitivity methods plays an important role in identifying these bacteria. Our goal was to identify these mutations using high-resolution melting (HRM) curve of DNA analysis.Methods: Resistance to mupirocin was identified using disc microdilution plate method in according to the Clinical and Laboratory Standards Institute (CLSI) guideline. mupA gene amplification in the isolates of methicillin-resistant Staphylococcus aureus was done using polymerase chain reaction (PCR). Then, analysis was performed using StepOne Software and HRM software. Sequencing was used as gold-standard method for confirming of the results.Findings: Out of 162 Staphylococcus aureus isolates, 83 (51.32%) were methicillin resistant. Among methicillin-resistant Staphylococcus aureus isolates, 47 (52.80 %) showed high level resistance to mupirocin carrying mupA. The most and lowest resistance was observed for penicillin (79.62%) and ceftazidime (6.17%), respectively. Moreover, all of multi-drug resistant (MDR) isolates were mupirucin resistant, too. Among mupirocin-resistant Staphylococcus aureus, wound samples were the most prevalent. Besides, isolates obtained from wound and blood demonstrated the highest mutation in A and G bases. Meaningful association was observed between the type of clinical sample and mutation rate, and resistance to mupirocin and methicillin (P < 0.05).Conclusion: Mupirocin-derived gene mutations provide multi-drug resistance in methicillin-resistant Staphylococcus aureus.
Keywords
Mupirocin
Methicillin resistance
Staphylococcus aureus
Mutation
DNA melting
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Journal of Isfahan Medical School
Volume 36, Issue 476 - Serial Number 476
March and April 2018
Pages 403-410

  • Receive Date 23 April 2018
  • Accept Date 06 April 2022